PLP不同肽段诱导EAE模型的对比研究

目的建立不仅与多发性硬化(multiple sclerosis，MS)临床表现、病理特征接近而且病程相似的较为理想的复发一缓解型实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis，EAE)模型。方法应用髓鞘蛋白脂质蛋白(proteolipid potein，PLP)多肽的两种免疫优势表位肽段PLP139-151和PLP178-191免疫雌性SJL／J小鼠，制作复发缓解型EAE(relapse remitting experimental autoimmune encephalomyelitis，RR—EAE)模型，观察其体重及神经功能评分的变化，应用HE、Luxol fas tblue髓鞘染色等方法观察模型的组织形态学改变。结果两种PLP肽段免疫的小鼠发病均具有缓解一复发的特点，出现明显的神经系统体征；小鼠发病时脑和脊髓组织显示明显的血管鞘形成、卫星现象和炎性细胞浸润以及脱髓鞘改变。结论PLP两种肽段均可诱发RR-EAE模型，这与临床MS的缓解一复发病程更相似，更能表现MS的临床特点，是研究MS的较为理想的动物模型。

Study of Various Kinds of Proteolipid Protein Inducing Experimental Autoimmune Encephalomyelitis

Objective To establish the Relapse remitting experimental autoimmune encephalomyelitis (RR-EAE) mouse model induced by proteolipid protein (PLP), which has imitate well clinical, course and histopathological alterations to multiple sclerosis (MS). Methods After injected with PLP_~139-151 or PLP_~178-191 , the body weight and neurological signs of each female SJL/J mouse were observed. The spinal and cerebral tissue specimens were stained by HE and Luxol fast blue. Results The model of EAE mouse induced by both two PLP peptides manifested significant neurological symptoms, signs and features of relapse and remitting. In spinal and ~cerebral tissue, obvious phenomena of perivascular ~inflammatory cuffing, satellitism, predominant perivascular inflammatory cell infiltration and demyelinated lesion could be found. Conclusions RR-EAE model induced by both two PLP peptides had more similarity to the relapse and remitting of MS. It might be a better model for the study of multiple sclerosis.