卡氏肺孢子虫感染大鼠肺肝脾微量元素的测定

目的 研究卡氏肺孢子虫(Pc)感染对大鼠肺、肝、脾组织中6种微量元素(Ca2+、Mg2+、Fe2+、Cu2+、Zn2+、Mn2+)的影响。 方法 30只SD大鼠随机分为实验组和对照组。实验组每只大鼠皮下注射地塞米松1 mg/次,每周2次,诱导Pc感染。10 wk后处死大鼠,检查Pc包囊,实验组分为Pc感染组和Pc阴性组。取肝、肺、脾组织,用原子吸收分光光度计测定其微量元素的变化。 结果 与Pc阴性组及对照组相比较,Pc感染组肺组织Zn2+含量明显低于对照组(P<0.05),Ca2+、Mg2+含量明显高于对照组(P<0.05),Fe2+、Cu2+、Mn2+含量变化不明显;感染组肝组织Zn2+含量明显低于对照组(P<0.05),Mg2+的含量增加(P<0.05),Ca2+、Fe2+、Cu2+、Mn2+的含量变化不明显;感染组脾脏中Zn2+、Cu2+的含量明显低于对照组(P<0.05),Ca2+、Mg2+、Fe2+、Mn2+的含量变化不明显。 结论 Pc感染大鼠肺、肝、脾组织微量元素的含量发生改变。

Analysis of Trace Elements in Lung,Liver and Spleen of Rats Infected with Pneumocystis carinii

Objective To study the effect of Pneumocystis carinii (Pc) infection on the level of six trace elements (Ca2+ , Mg2+ , Fe2 + , Cu2 + , Zn2+ , Mn2+ ) in the lung, liver and spleen of the rats. Methods 30 rats were randomly divided into two groups:20 rats in the experimental group and 10 in the control group. Each rat in the experimental group was injected subcutaneously with dexamethasone (1 mg per rat) twice a week. All rats in the experiment group (Pc infected and PC negative) and the control group were killed to obtain lungs, livers and spleens after 10 weeks and the atomic absorption method was used for element analysis. Results Compared with the Pc negative group and the control group, the level of Zn2+ in the lung in Pc infected group was significantly reduced (P<0.05). The amount of Ca2+ and Mg2+ in the infected rats were higher than that of the control (P<0.05). No difference was determined in the content of Fe2+ , Cu2+ and Mn2+ among the Pc infected group, the Pc negative group and the control group. The level of Zn2+ in liver in the Pc infected group was significantly reduced (P<0.05). The amount of Mg2+ in the Pc infected rats was higher than that of the control (P<0.05), but no difference was found in the content of Ca2+ , Fe2+ , Cu2+ and Mn2 + among the groups. The level of Zn2+ and Cu2+ in spleen in Pc infected group was significantly reduced (P < 0. 05 ) , and no difference was found in the content of Ca2+ , Mg2+ , Fe2+ and Mn among the three groups. Conclusion Pneumocystis carinii infection might play a role in the changes of trace elements in the lung, liver and spleen of rats.