改进型左侧精索静脉曲张对大鼠睾丸酶及增殖细胞核抗原的影响

Objective To evaluate the effects of improved left varicocele (ELV) on testis enzymes and proliferating cell nuclear antigen (PCNA) in rats. Methods ELV models of SD rats were established by the improved method in 21 SD rats, and another 15 were included in a sham operation group as controls. At the 3rd month, the activity of LDH, SDH and G6PDH was measured by colorimetry. The concentration of the PCNA was determined by enzyme-linked immunosorbent assay ( ELISA). Results The activity of LDH and G6PDH (8. 17 ± 3. 47), (34. 00 ± 16. 29) U/mg in left testes of experimental group was reduced remarkably as compared with that in right testes (12.69 ±3.97), (78.03 ± 25. 28) U/mg and control group (11. 98 ± 2. 26) , (54. 88 ± 20. 87) U/mg (P < 0. 05). The activity of SDH was reducd remarkably in left testes of experimental group ( 1. 22 ±0. 41 ) U/mg as compared with that in control group (1.74 ± 0.43) U/mg (P < 0.05), but there was no significant difference between the left and right testes ( 1.62 ±0. 56) U/mg in experimental group. The concentration of PCNA was reduced in left testes of experimental group (669. 10 ±205. 39) mU/ml as compared with that in right testes (800. 81 ± 172. 02) mU/ml and control group (776. 81 ± 231. 72) mU/ml, but there was no significant difference (P > 0. 05). Conclusion The reduction of LDH, SDH and G6PDH and the changes of PCNA in improved ELV may play an important role in the development of subfertility.

Effects of improved varicocele on testis enzymes and proliferating cell nuclear antigen in rats