| 荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义 |
| |
| 引用本文： | 徐盈斌,赵李平,柯昌能,舒斌,唐锦明,谢举临,祁少海,刘旭盛,利天增.荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义[J].中华实验外科杂志,2010,28(12):694-696. |
| |
| 作者姓名： | 徐盈斌 赵李平 柯昌能 舒斌 唐锦明 谢举临 祁少海 刘旭盛 利天增 |
| |
| 作者单位： | 中山大学附属第一医院烧伤外科,广州,510080;安徽省立医院整形烧伤科;汕头大学医学院第二附属医院烧伤科; |
| |
| 基金项目： | 广东省自然科学基金资助项目创伤、烧伤与复合伤国家重点实验室开放基金资助项目 |
| |
| 摘 要： | Objective To discuss the influence of aerosol bioelectricity on the expression of interleukin (IL) -8 and IL-10 in wound healing of burned rats. Methods The deep Ⅱ degree scalding models were established in Sprague Dawley (SD) rats. Rats were randomly divided into experimental group (n1 =20) and control group (n2 =20). The rats in experimental group were treated with aerosol bioelectricity.Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7. 00 ± 1. 15 days, and that in control group was 9. 00 ± 1. 34 days. IL-8 and IL-10 were observed mainly in polylmorphonuclear and mononuclear cells in both experimental and control groups on the 1 st day. On the third day, fibroblasts abounded, IL-8 expression was increased evidently and reached a peak. The peak value (6. 73 ± 1. 36) in experimental group was lower significantly than that in control group ( 2. 85 ± 0. 72, P ＜ 0. 01). From the 5th to 11th day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group (1. 24 ±0. 15) and control group (5. 69 ± 1. 32) on the 3rd day. IL-10 expression was declined gradually from the 5th to 11th days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to 11th days post-scalding (P＜0. 01). On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly , and there was negative relationship between them (r = - 0. 862, P ＜ 0. 01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.
|
| 关 键 词： | 气溶胶 烧伤 创面愈合 白细胞介素 |
Influence of aerosol bioelectricity on the expression of interleukin-8 and interleukin-10 in burned rats' wound healing |
| |
| XU Ying-bin,ZHAO Li-ping,KE Chang-neng,SHU Bin,TANG Jin-ming,XIE Ju-lin,QI Shao-hai,LIU Xu-sheng,LI Tian-zeng.Influence of aerosol bioelectricity on the expression of interleukin-8 and interleukin-10 in burned rats' wound healing[J].Chinese Journal of Experimental Surgery,2010,28(12):694-696. |
| |
| Authors: | XU Ying-bin ZHAO Li-ping KE Chang-neng SHU Bin TANG Jin-ming XIE Ju-lin QI Shao-hai LIU Xu-sheng LI Tian-zeng |
| |
| Abstract: | |
| |
| Keywords: | AerosolBurnWound healingInterleukin |
|
|
