白藜芦醇诱导急性T淋巴母细胞白血病Jurkat细胞凋亡

目的：探讨白藜芦醇抑制急性T淋巴母细胞白血病Jurkat细胞增殖、引发S期阻滞和诱导凋亡的作用。方法：白藜芦醇体外处理培养的Jurkat细胞后，采用MTT法测定细胞活力，Wrigh-Giemsa染色、Hoechest 33258/PI荧光染色和透射电镜观察Jurkat细胞的形态学改变；流式细胞术分析细胞周期。结果：白藜芦醇0.2 mmol·L^-1处理组的抑制Jurkat细胞增殖率达64.01%，并具有剂量和时间依赖性。白藜芦醇处理细胞24 h后可见凋亡形态学改变，Hoechest 33258/PI染色显示白藜芦醇处理组细胞部分细胞核呈亮蓝色，随培养时间延长，亮蓝色荧光染色细胞数量逐渐增多。Wrigh-Giemsa染色和透射电镜观察发现部分细胞体积缩小、染色质浓缩及边集等现象的出现。流式细胞术检测表明，0.05 mmol·L^-1白黎芦醇处理48 h组62.57％的细胞被阻滞于细胞周期S期，亚二倍体细胞数量12.01%，未加药对照组细胞亚二倍体细胞数量2.05%。结论：白藜芦醇可以抑制Jurkat细胞的增殖，引起细胞周期的S期阻滞，并诱导其发生凋亡

Resveratrol-induced apoptosis of Jurkat cell line in acute T lymphoblast leukemia

Objective To investigate the effect of resveratrol on proliferation inhibition,cell cycle arrest and apoptosis of Jurkat cell line in acute T lymphoblast leukemia.Methods MTT assay was used to determine the cell vitality.Wright-Giemsa,Hoechest 33258/PI staining and transmission electron microscope technique were used to detect the apoptosis status of Jurkat cells.The cell cycle arrest was analyzed by flow cytometry.(Results Resveratrol) had 64.01% inhibitory rate on the growth of Jurkat cells at 0.2 mmol·L~(-1) and inhibited the growth of Jurkat cells in dose-and time-dependent manner.24 h after treated with resveratrol,the typical features of apoptosis were observed under light and electron microscope in all treatment groups.Some nuclei showed bright blue under fluorescence microscope in the resveratrol-treated Jurkat cells,and the number of cells with bright blue fluorescence increased with time.Nuclei condensation and fragmentation were observed.Cell shrinkage,chromatin condensation,and marginalization were found by Wrigh-Giemsa staining and transmission electron microscope technique.By flow cytometry,62.57% of the cells were arrested at the S phase after exposured to(0.05 mmol·L~(-1)) resveratrol for 48 h,the rate of apoptotic cells to total cells was 12.01% in 0.05 mmol·L~(-1) treatment groups,and that in the control groups was 2.05%. Conclusion Resveratrol can inhibit the proliferation,cause S-phage arrest and induce the apoptosis of Jurkat cells.