四川省2006年B型流感病毒抗原性及基因特性研究

[目的]阐明2006年四川省流行的B型流感病毒血凝素抗原性及其基因变异情况。[方法]对2006年分离的B型毒株进行单向血凝抑制实验,再将病毒株进行血凝素基因HA1区核苷酸序列测定并推导出其氨基酸序列,然后进行基因进化特性分析。[结果]2006年四川省人群中同时流行着B型Victoria系和Yamagata系毒株。Victoria系毒株与B/Hongkong/330/2001比较,病毒单向血凝抑制效价均有4倍以上差异,并且在HA1区发生8个氨基酸替换,在197位增加一个糖基化位点。Yamagata系毒株与B/Shanghai/361/2002比较,病毒单向血凝抑制效价均有4倍以上差异,并且在血凝素基因HA1区有7个氨基酸位点发生改变,在196位增加一个糖基化位点。[结论]四川省2006年B型流感病毒的抗原性与B/Hongkong/330/2001、B/Shanghai/361/2002相比已经发生了变化。

STUDY ON ANTIGENIC CHARACTERISTICS AND GENETIC VARIATIONGS OF INFLUENZA B VIRUS CIRCULATING IN SICHUAN PROVINCE IN 2006

[Objective] To identify the antigenic characteristics and genetic variations of hemagglutinin genes from Influenza B virus, which circulated in Sichuan Province in 2006. [ Methods] The single-way Hemagglutination Inhibition Tests was used to test the antigenic characteristics of the isolated strain of Influenza B virus. The nucleotide sequence of HA1 domain was tested and the amino acid sequence was deduced, then gene conversion levels were assessed. [ Results] The Yamagata- like and Victoria-like viruses co-circulated in 2006. As for Victoria-like virus, not only 4 folds greater HI fiter was obtained, but also 8 amino acid mutations in HA1 domain and one more glyeosylation site at 196 were found. As for Yamagata-like virus, compared with B/Shanghai/361/2002, the same 4 folds higher HI titer, 7 amino acid mutations identified in HA1 domain and one more glycosylation site at 197 were found. [ Conclusions] Compared with B/Hongkong/330/2001and B/Shanghai/361/ 2002, Influenza B viruses discovered in Siehuan Province in 2006 has had antigenic variation aheady.