esp基因存在与粪肠球菌耐药的相关性分析

目的研究粪肠球菌临床株表面蛋白基因(esp)的流行情况及esp基因存在状况与粪肠球菌耐药的相关性。方法用PCR法扩增粪肠球菌esp基因并克隆、测序;用琼脂稀释法检测5种常用抗菌药物对粪肠球菌临床株的最低抑菌浓度(MIC)。结果61株粪肠球菌中,esp基因阳性率为42.6%;氨苄西林、环丙沙星、高浓度庆大霉素MIC>500 mg/L,红霉素耐药粪肠球菌中,esp阳性率分别为33.3%、54.8%、70.6%、51.0%;敏感株中esp阳性率分别为43.6%、30.0%、7.4%、8.3%。结论esp基因的存在状况与粪肠球菌对氨苄西林耐药无明显相关性,而与环丙沙星、红霉素、高浓度庆大霉素耐药有显著相关性;esp基因有可能成为粪肠球菌耐药株的分子表面标志物。

Expression Situation of esp Gene and Analysis of Relativity with Enterococcus faecalis Resistance

OBJECTIVE To study the expression situation of esp gene and the relativity between esp gene and resistance of Enterococcus faecalis derived-infection.METHODS The E.faecalis esp gene was amplified by PCR,then it was cloned and sequenced;the minimum inhibitory concentration(MIC) of five antimicrobicals against E.faecalis was determined by agar dilution method.RESULTS The positive rate of esp gene in 61 strains of E.faecalis was 42.6%;the positive rate of esp gene in the resistant strains against ampicillin,ciprofloxacin,high-level gentamicin,and erythromycin was 33.3%,54.8%,70.6%,and 51.0%,respectively,and the positive rate of esp gene in the susceptible strains against above 4 antibacterials was 43.6%,30.0%,7.4%,and 8.3%,respectively.CONCLUSIONS Incidence of esp gene does not correlate with the resistance of E.faecalis against ampicillin,but clearly correlate with the resistance of E.faecalis against ciprofloxacin,high-level gentamicin,and erythromycin;esp could become a marker of high-level gentamicin against E.faecalis derived-infection.