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脑脊液细胞学迈-格-姬染色在隐球菌脑膜炎诊断中的价值
引用本文:樊新红,冯国栋,杨毅宁,代文,赵钢.脑脊液细胞学迈-格-姬染色在隐球菌脑膜炎诊断中的价值[J].国际神经病学神经外科学杂志,2013,40(3):220-222.
作者姓名:樊新红  冯国栋  杨毅宁  代文  赵钢
作者单位:1. 解放军第四军医大学西京医院神经内科,陕西省西安市710032;中国兵器工业521医院神经内科,陕西省西安市710065
2. 解放军第四军医大学西京医院神经内科,陕西省西安市,710032
摘    要:目的 探讨脑脊液细胞学迈-格-姬(MGG)染色在隐球菌脑膜炎诊断中的价值.方法 对笔者所在医院诊治的48例隐球菌脑膜炎患者脑脊液涂片墨汁染色、细胞学MGG染色检查结果及诊断进行分析.结果 48例隐脑患者首次脑脊液常规墨汁染色发现隐球菌33例,首次检出率为68.8%;MGG染色发现46例,首次检出率为95.8%.MGG染色诊断隐球菌脑膜炎的敏感度高于墨汁染色,差别有统计学意义(P<0.05).结论 脑脊液MGG染色检查对隐球菌的检出率可达95.8%,且可在脑脊液细胞学检查时一次完成,无需特殊染色,可作为中枢神经系统感染患者常规脑脊液细胞学检查.

关 键 词:脑脊液  脑膜炎  隐球菌  迈-格-姬染色  墨汁染色  诊断
收稿时间:2013/4/2 0:00:00
修稿时间:2013/5/30 0:00:00

Diagnostic value of May-Grunwald-Giemsa staining of cerebrospinal fluid in patients with cryptococcal meningitis
FAN Xin-Hong,FENG Guo-Dong,YANG Yi-Ning,DAI Wen,ZHAO Gang.Diagnostic value of May-Grunwald-Giemsa staining of cerebrospinal fluid in patients with cryptococcal meningitis[J].Journal of International Neurology and Neurosurgery,2013,40(3):220-222.
Authors:FAN Xin-Hong  FENG Guo-Dong  YANG Yi-Ning  DAI Wen  ZHAO Gang
Institution:Department of Neurology, Xijing Hospital, the Fourth Military Medical University, Xi''an 710032, China
Abstract:

Objective To evaluate the diagnostic value of May-Grunwald-Giemsa (MGG) staining of cerebrospinal fluid in patients with cryptococcal meningitis. Methods The cerebrospinal fluid smears of 48 patients with cryptococcal meningitis in Xijing Hospital were subjected to India ink staining and MGG staining to detect Cryptococcus, and the diagnostic values of ink staining and MGG staining were compared according to the detection results. Results The India ink test was positive in 33 (68.8%) of 48 patients, while the MGG staining was positive in 46 (95.8%) of the 48 patients. MGG staining was significantly more sensitive than ink staining for diagnosis of cryptococcal meningitis (P<0.005). Conclusions MGG staining increases the Cryptococcus detection rate up to 95.8% and can be finished in one step without need for special staining. It can be used as a conventional means of cerebrospinal fluid cytology in patients with central nervous system infection.

Keywords:cerebrospinal fluid  meningitis  cryptococcal  May-Grunwald-Giemsa staining  ink staining  diagnosis
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