碱性成纤维细胞生长因子增强淋巴因子激活的杀伤细胞对人膀胱 …

目的 探讨白细胞介素Ⅱ（ＩＬ－２）和碱性成纤维细胞生长因子（ｂＦＧＦ）对膀胱癌患者淋巴因子激活的杀伤细胞（ＬＡＫ细胞）的作用。方法 用细胞计数观察不同浓度ｂＦＧＦ对ＬＡＫ细胞增殖的影响，以膀胱癌细胞系ＥＪ及新鲜分离患者自体肿瘤细胞（ＢＴＣ）为靶细胞，用ＭＴＴ法测定ＬＡＫ细胞对膀胱癌细胞的细胞毒作用，结果 虽然外周血单核细胞（ＰＢＭＣ）的增殖可被ｂＦＧＦ５μｇ．Ｌ＾－１所抑制，ＩＬ－２所诱导的ＬＡＫ

Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells.

AIM: To study the effects of basic fibroblast growth factor (bFGF) on the proliferation of lymphokine-activated killer (LAK) cells from patients with bladder cancer and LAK cells cytolysis against bladder tumor cells. METHODS: LAK cell proliferation was assayed in the presence of various concentrations of bFGF combined with interleukin-2 (IL-2) by cell count. Cytotoxicity of LAK cells against bladder cancer cell line EJ cells and bladder tumor cells (BTC) from patients was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. RESULTS: The proliferation of peripheral blood monocytes (PBMC) was inhibited by bFGF 5 micrograms.L-1. bFGF did not affect the stimulation of LAK cells induced by IL-2. The LAK cell numbers in the combination of IL-2 with bFGF were not significantly different compared with that treated with IL-2 alone. bFGF enhanced cytotoxicity of LAK cells against bladder cancer cell line EJ cells or BTC, respectively. CONCLUSION: Although the proliferation of PBMC was inhibited by bFGF, bFGF increased LAK cell cytotoxicity against bladder neoplasm cells.