Erum-induced changes in electrophoretic mobility of creatine phosphokinase isoenzymes? |
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| Authors: | PM Bayer Th Gergely F Gabl E Deutsch |
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| Institution: | Department for Clinical Chemistry, 1st Medical Clinic of the University of Vienna, Lazarettgasse 14, A-1090 ViennaAustria |
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| Abstract: | BB isoenzyme of creatine phosphokinase (CK-BB) obtained from human brain-extract changes its electrophoretic mobility after incubation in human serum at 37° C. No change of electrophoretic mobility of CK-BB is observed after incubation in isotonic saline. We have shown by means of immunoprecipitation with specific antibodies that the structure of CK-BB is not changed. These findings are supported by other authors and make the diagnostic value of electrophoretic separation of CK isoenzymes doubtful as after a 3-h incubation CK-BB migrates similarly to CK-MB and consequently may be misinterpreted. |
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| Keywords: | To whom correspondence and reprint requests should be addressed |
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