双黄乳膏不同皮肤体外扩散的实验研究

目的：研究两种氮酮（Azone）含量的双黄乳膏中盐酸小檗碱在不同皮肤的经皮扩散情况并对结果进行分析比较。方法：使用智能透皮试验仪对含氮酮1%（A）和4%（B）的两种双黄乳膏进行体外经皮扩散实验,测定累积透过量和经皮扩散速率。结果：鼠皮（背皮、腹皮）的累积透过量及经皮扩散速率均明显高于蛇蜕（P〈0.01）。对于蛇蜕,A乳膏累积透过量及经皮扩散率高于含B乳膏（P=0.037,0.028）;对于鼠皮,则累积透过量差异无统计学意义,经皮扩散率B乳膏反而高于A乳膏（P=0.046）。结论：采用不同皮肤会造成实验结果的不同。用蛇蜕筛选氮酮的用量,灵敏度高,干扰因素少,结果稳定。

Experimental Study on Transdermal Diffusion of Shanghuan Cream in Vitro on Different Skins

Objective： To study the transdermal diffusion of berberine hydrochloride in Shanghuang Cream with two different Azone contents on different skins and to compare and analyze the effect. Methods： Intelligent transdermal test instrument was used to determine the cumulative permeating amounts of berberine hydrochloride in two kinds of Shanghuang Cream with 1% azone（ Cream A） and 4% azone（ Cream B）. Then diffusion rate was calculated. Results： The cumulative permeating amounts and transdermal diffusion rate on mice skin,including skin of back and abdomen,were significantly higher than those on snake slough（ P〈0. 01）. On snake slough,the cumulative permeating amounts and transdermal diffusion rate of berberine hydrochloride in cream A was significantly higher than cream B（ P = 0. 037,0. 028）. The difference of the cumulative permeating amounts between two creams was not significant on mice skin,but the transdermal diffusion rate of cream B was significantly higher than that of cream A on mice skin of back（ P = 0. 046）. Conclusion：Different skin shows different result. Snake slough was superior to mice skin in optimizing Azone content because of its high sensitivity,less interferences and excellent stability.