五种基因的蛋白分子表达与鼻咽癌放疗疗效的关系

目的探讨上皮钙黏附蛋白（E-cadherin）、黏附分子CD44H、基质金属蛋白酶-3（matrix metalloproteinase-3，MMP-3）、肿瘤转移抑制基因nm23H1和血管内皮生长因子（vascular endothelial growth factor，VEGF）的表达与鼻咽癌放疗疗效的关系，并分析其预测鼻咽癌疗效的价值。方法应用免疫组织化学SP法检测62例鼻咽癌组织中E-cadherin、CD44H、MMP-3、nm23H1和VEGF的蛋白分子表达水平。结果CD44H（χ^2=18．739，P=0．028）和VEGF（χ^2=18．523，P=0．030）的表达与鼻咽癌放疗后近期疗效密切相关，表达增强则近期疗效下降。CD44H和nm23H1低表达组的3年生存率分别为65．5％、45．5％，5年生存率分别为47．3％、22．7％；高表达组的3年生存率分别为54．6％、75．9％，5年生存率分别为27．8％、53．2％；CD44H（χ^2=7．31，P=0．0069）和nm23H1（χ^2=15．64，P=0．0001）两表达组的生存率差异有统计学意义。结论检测CD44H和VEGF的表达可能作为预测鼻咽癌放疗后近期疗效的指标，检测CD44H和nm23H1的表达可能作为预测鼻咽癌放疗后远期疗效的指标。

Expressions and correlation with radiotherapy's effect of five kinds of genes in nasopharyngeal carcinoma

Objective To investigate the expressions of E-cadherin, CD44H, matrix metalloproteinase-3 (MMP-3) , nm23Hl and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma and its relationship to the effect of radiotherapy. Furthermore, to analyze the predict value of radiotherapy effect. Methods The expressions of E-cadherin, CD44H, MMP-3, nm23H1 and VEGF in 62 patients with nasopharyngeal carcinoma were determined by immunohistochemical SP method. There were 62 patients (17 to 70 years old) with nasopharyngeal carcinoma which were treated by radiotherapy from March 1995 to October 1995 and the period of follow-up had full 10 years. Results The expressions of CD44H( x2 = 18. 739 ,P =0. 028) and VEGF(x2 = 18. 523 ,P = 0. 030) were closely related with short-term effect after radiotherapy in nasopharyngeal carcinoma The short-term effect was descent along with enhancement of their expressions. In the group of low expressions in CD44H and nm23H1, 3-year overall survival rate were 65. 5% and 45. 5% , and 5-year overall survival rate were 47. 3% and 22. 7% . In the group of high expressions in CD44H and nm23H1, 3-year overall survival rate were 54. 6% and 75. 9% , and 5-year overall survival rate were 27. 8% and 53. 2% . There were respectively significant difference between two group of expressions in CD44H (x2= 7. 31, P= 0. 0069) and nm23Hl ( x2= 15. 64, P = 0. 0001 ). Conclusions These findings indicated that to detect the expressions of CD44H and VEGF gene may predict short-term effect of radiotherapy. Furthermore, to detect the expressions of CD44H and nm23H1 gene may predict long-term effect of radiotherapy in nasopharyngeal carcinoma.