| 外排泵抑制剂对幽门螺杆菌多重耐药性的影响 |
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| 引用本文： | 张展,刘志强,郑鹏远,唐芙爱. 外排泵抑制剂对幽门螺杆菌多重耐药性的影响[J]. 世界华人消化杂志, 2010, 18(3) |
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| 作者姓名： | 张展 刘志强 郑鹏远 唐芙爱 |
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| 作者单位： | 郑州大学第二附属医院消化内科,河南省,郑州市,450014 |
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| 基金项目： | 河南省杰出青年基金资助项目 |
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| 摘 要： | 目的:探索多种外排泵抑制剂对H.pylori多重耐药性的影响.方法:临床分离培养H.pylori,对分离敏感株进行氯霉素耐药性诱导,筛选出多重耐药(MDR)株;琼脂二倍稀释法对敏感菌株和MDR株进行9种抗生素的药敏试验,分别加入外排泵抑制剂碳酰氰基-对-氯苯腙(CCCP)、利血平、泮托拉唑后再次进行药敏试验,比较分析外排泵抑制剂对H.pylori耐药性的影响;同样方法检测泮托拉唑、雷贝拉唑、奥美拉唑、兰索拉唑和埃索美拉唑对MDR-H.pylori株抗生素最小抑菌浓度(MIcs)值的影响.结果:诱导出4株MDR-H.pylori;CCCP及泮托拉唑能够部分恢复MDR-H.pylon株对抗生素的敏感性,而利血平对MDR-H.pylori耐药性无明显影响;这三种外排泵抑制剂对敏感株的MICs值影响不大;5种质子泵抑制剂中,雷贝拉唑能使甲硝唑和阿莫西林的MIC值分别降低至1/4和1/3,泮托拉唑能使其均降低至1/2左右.结论:外排泵抑制剂能够在体外提高多重耐药H.pylori株的药物敏感性;在所有质子泵抑制荆中雷贝拉唑对降低MDR-H.pylori对抗生素的耐药性作用最强,其次为泮托拉唑.
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| 关 键 词： | 外排泵抑制剂 幽门螺杆菌 多重耐药 质子泵抑制剂 荧光实时定量PCR |
Effects of efflux pump inhibitors on the multidrug resistance of Helicobacter pylori |
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| Zhan Zhang,Zhi-Qiang Liu,Peng-Yuan Zheng,Fu-Ai Tang. Effects of efflux pump inhibitors on the multidrug resistance of Helicobacter pylori[J]. World Chinese Journal of Digestology, 2010, 18(3) |
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| Authors: | Zhan Zhang Zhi-Qiang Liu Peng-Yuan Zheng Fu-Ai Tang |
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| Affiliation: | Zhan Zhang,Zhi-Qiang Liu,Peng-Yuan Zheng,Fu-Ai Tang,Department of Gastroenterology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,Henan Province,China |
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| Abstract: | AIM:To evaluate the effects of efflux pump inhibitors(EPIs) on the multidrug resistance(MDR) of Helicobacter pylori(H.pylori).METHODS:H.pylori strains were isolated and cultured on Brucella agar plates containing 10% sheep blood.The MDR of H.pylori strains was induced with chloramphenicol.The susceptibility of multidrug-resistant H.pylori strains and their parental strains to nine antibiotics was assessed by agar dilution test in the presence and absence of EPIs such as CCCP, reserpine and pantoprazole.The ... |
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| Keywords: | Efflux pump inhibitor Helicobacter pylori Multidrug resistance Proton pump inhibitor Real-time fluorescence quantitative PCR |
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