| 丝/苏氨酸蛋白激酶Plk1和转录阻遏子NAC1存在相互作用 |
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| 引用本文： | 熊福银,温叶飞,林艳丽,周艳荣,施庚寿,田利源,曾强成,韩正滨,邓继先,陈红星. 丝/苏氨酸蛋白激酶Plk1和转录阻遏子NAC1存在相互作用[J]. 军事医学科学院院刊, 2007, 31(4): 305-307 |
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| 作者姓名： | 熊福银 温叶飞 林艳丽 周艳荣 施庚寿 田利源 曾强成 韩正滨 邓继先 陈红星 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 摘 要： |
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| 关 键 词： | Plk1 NAC1 酵母菌 双杂交系统技术 相互作用 苏氨酸蛋白激酶 转录 阻遏子 存在 相互作用的分子 serine Interaction repressor 发展 发生 神经系统疾病 肿瘤 发育分化 细胞亚定位 酵母双杂交技术 空间 nucleus 结果 分析 免疫共沉淀 |
| 文章编号： | 1000-5501（2007）04-0305-03 |
| 修稿时间： | 2007-01-26 |
Interaction of serine/threonine protein kinase Plk1 and transcriptional repressor NAC1 |
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| XIONG Fu-Yin,WEN Ye-Fei,LIN Yan-Li,ZHOU Yan-Rong,SHI Geng-Shou,TIAN Li-Yuan,ZENG Qiang-Cheng,HAN Zheng-Bin,DENG Ji-Xian,CHEN Hong-Xing. Interaction of serine/threonine protein kinase Plk1 and transcriptional repressor NAC1[J]. Bulletin of the Academy of Military Medical Sciences, 2007, 31(4): 305-307 |
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| Authors: | XIONG Fu-Yin WEN Ye-Fei LIN Yan-Li ZHOU Yan-Rong SHI Geng-Shou TIAN Li-Yuan ZENG Qiang-Cheng HAN Zheng-Bin DENG Ji-Xian CHEN Hong-Xing |
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| Affiliation: | Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract: | Objective: To investigate the protein partners interacted with the polo-like kinase 1(Plk1).Methods: The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,one of the candidate molecules was found to be nucleus accumbems-1(NAC1).The subcellular location of NAC1 and Plk1 was determined by fluorescent microscopy.The interaction of Plk1 and NAC1 was confirmed by co-immunoprecipitation in vivo and GST-pulldown in vitro.Results and Conclusion:The data indicated that Plk1 could interact with NAC1 both in vitro and in vivo. The interaction between Plk1 and NAC1 may play an important role in the cellular development,particularly in the onset and development of the oncological and nervous system diseases. |
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| Keywords: | Plk1 NAC1 yeasts two-hybrid system techniques interaction |
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