| 免疫金银染色法在蛋白质微阵列检测TORCH感染的实验研究 |
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| 引用本文： | 余章斌 蒋犁 张春秀 唐祖明 陆祖宏 蒋涛. 免疫金银染色法在蛋白质微阵列检测TORCH感染的实验研究[J]. 临床检验杂志, 2006, 24(2): 125-127 |
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| 作者姓名： | 余章斌 蒋犁 张春秀 唐祖明 陆祖宏 蒋涛 |
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| 作者单位： | 东南大学临床医学院,南京,210009;东南大学分子与生物电子国家重点实验室,南京,210096;南京医科大学附属南京妇幼保健院,南京,210029 |
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| 基金项目： | 江苏省自然科学基金(BK2003056)，国家自然科学基金预研项目(XJ0490166)资助 |
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| 摘 要： | 目的研制基于免疫金银染色法的TORCH检测蛋白质微阵列。方法将TORCH抗原点样于已经被琼脂糖膜修饰的玻片表面,与待测血清和胶体金标记的抗人IgM(μ链)孵育,用银增强微阵列信号,肉眼观察或普通的平板扫描仪记录结果。以不同浓度的纯化人IgM制备标准曲线。结果蛋白质微阵列的灰度质和IgM的浓度呈线性关系。60份待测血清与ELISA法同时检测,诊断符合率为93.3%。结论该法具有高信息通量、低成本、制备与检测快速简单的优点。
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| 关 键 词： | 免疫金银染色法 蛋白质 微阵列 TORCH感染 |
| 文章编号： | 1001-764X(2006)02-0125-03 |
| 收稿时间： | 2005-07-06 |
| 修稿时间： | 2005-12-10 |
Experimental study on detection of TORCH infection using protein microarrays with immuno-gold-silver staining method |
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| YU Zhangbin,JIANG Li,ZHANG Chunxiu,TANG Zuming,LU Zuhong,JIANG Tao. Experimental study on detection of TORCH infection using protein microarrays with immuno-gold-silver staining method[J]. Chinese Journal of Clinical Laboratory Science, 2006, 24(2): 125-127 |
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| Authors: | YU Zhangbin JIANG Li ZHANG Chunxiu TANG Zuming LU Zuhong JIANG Tao |
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| Affiliation: | 1. School of Clinical Medicine ,Southeast University ,Jiangsu, Nanjing 210009, China;2, National Laboratory for Molecular and Biomolecular Electronics, Southeast University ,Jiangsu, Nanjing 210096, China;3. Nanjing Maiernity and Child Health care Hospital, Affiliated Hospital of Nanjing Medical University, Jiangsu, Nanj ing 210029, China |
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| Abstract: | Objective To study a protein microarray based on immuno-gold-silver staining method for the detection of TORCH infection.Methods The antigens were printed on glass slide modified with agarose films.The gold nanoparticles were introduced to the microarray by specific binding of gold-conjugated antibodies and then coupled with silver enhancement to produce black image of microarray spots,which could be easily imaged with a simple flatbed scanner or even naked eyes.Results We created the human IgM dose-response curves which had a good linear correlation between the gray level and the sample concentration.A concordance of 93.3% was obtained between protein microarray and ELISAs in the detection for sera.Conclusions The technique of protein microarray is a rapid,simple,low-cost and high-throughput approach which is displaying more practical and broad applications. |
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| Keywords: | immuno-gold-silver staining protein microarray TORCH infection |
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