| 羊心房钠尿因子的提取与部分纯化和生物学活性 |
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| 引用本文： | 姜玲玲,赵工,房世荣,刘庆辉. 羊心房钠尿因子的提取与部分纯化和生物学活性[J]. 河北医科大学学报, 1987, 0(2) |
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| 作者姓名： | 姜玲玲 赵工 房世荣 刘庆辉 |
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| 作者单位： | 河北医学院基础医学研究所，河北医学院基础医学研究所，河北医学院基础医学研究所，河北医学院基础医学研究所 |
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| 摘 要： | 本文报道了羊心房钠尿因子的提取、部分纯化过程和生物学活性。结果表明:羊心房粗提物经超滤,Sephadex G-15、Sephadex G-75凝胶柱层析分离得到的部分纯化的羊心房提取物有较高的利尿钠尿活性。给麻醉大鼠静脉注射小剂量(350μg蛋白/Kg体重)部分纯化的羊心房提取物后,尿量,尿钠、尿钾分别增加2.17倍,4.95倍和0.81倍。这种利尿和钠尿效应与剂量有明显的依赖关系。
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| 关 键 词： | 心房钠尿因子 分离和纯化 钠尿因子 生理学 |
ISOLATION, PARTIAL PURIFICATION AND BIOACTIVITY OF ATRIAL NATRIURETIC FACTOR IN SHEEP |
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| Jiang Lingling Zhao Gong Fang Shirong Liu Qinghui Institute of Basic Medicine. ISOLATION, PARTIAL PURIFICATION AND BIOACTIVITY OF ATRIAL NATRIURETIC FACTOR IN SHEEP[J]. Journal of Hebei Medical University, 1987, 0(2) |
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| Authors: | Jiang Lingling Zhao Gong Fang Shirong Liu Qinghui Institute of Basic Medicine |
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| Affiliation: | Jiang Lingling Zhao Gong Fang Shirong Liu Qinghui Institute of Basic Medicine |
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| Abstract: | Chemical extraction and purification procedures for sheep atrial natriuretic factor (ANF) were described. The partial purification was obtained after Sephadex G-15 and Sephadex G-75 gel filtration. By injecting the partial purified ANF of sheep atrium into rats, the urine volume, ths urinary sodium and potassium excretions were increased by 217%, 4S5% and 81%, respectively The above-mentioned effects were dose-dependent. |
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| Keywords: | atrial natriuretic factor isolation and purification ANF physiology |
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