Development and characterization of ten microsatellite loci for the eastern spadefoot toad, Scaphiopus holbrookii |
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| Authors: | Matthew R. Erickson David E. Scott Kenneth L. Jones Cris Hagen Stacey L. Lance |
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| Affiliation: | 1. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA
2. Georgia Genomics Facility, University of Georgia, Athens, GA, 30602, USA
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| Abstract: | We isolated and characterized 10 microsatellite loci from the eastern spadefoot toad, Scaphiopus holbrookii. Loci were screened in 24 individuals from two schools of tadpoles in a single isolated wetland in South Carolina, USA. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity values ranged from 0.043 to 0.298. These new loci provide tools for examining the landscape genetics of a species facing continued destruction of its breeding habitat (small isolated wetlands) as well as fragmentation of upland life zones. |
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