Ultrastructural morphometry of normal human dermal-epidermal junction. The influence of age, sex, and body region on laminar and nonlaminar components

To obtain baseline data for future studies on such processes as wound healing, carcinogenesis, and blistering, a morphometric analysis of the dermal-epidermal junction was undertaken on normal skin from 3 or 4 standard sites on the arm and leg of 12 subjects aged 20-60 years. Lamina densa was thinner in females than in males (p less than 0.01) but no sex difference was apparent for lamina lucida. Both laminar elements were thinner beneath melanocytes than beneath keratinocytes. Sex, age, and body region had no apparent influence on numbers of hemidesmosomes or basal cell plasmalemmal vesicles, nor was there a significant variation of these structures among individuals. Numbers of dermal microfibril bundles diminished with age (p less than 0.01). Anchoring fibril counts varied widely both among individuals (p less than 0.025) and within the same subject; there were fewer in the upper arm compared with different parts of the leg (p less than 0.005). These results emphasize the importance of appropriate controls in studies of physiologic and pathologic conditions involving the dermal-epidermal junction.