苦荞壳提取物对高脂饲料诱导的大鼠脂肪肝的预防作用

目的: 观察苦荞壳提取物对高脂饲料诱导的大鼠脂肪肝的预防作用,探讨其机制. 方法: 雄性Wistar大鼠50只,按体质量随机分为普通饲料组、高脂模型组、苦荞壳提取物高剂量组(0.825 g/kg)、低剂量组(0.165 g/kg)和力平之对照组(0.067 g/kg). 于实验后第12 wk测定大鼠肝脏Ch, Tg, MDA浓度和SOD, GPX活力,并观察大鼠肝组织病理变化. 结果: 高脂模型组大鼠肝脏Ch, Tg, MDA含量显著高于普通饲料组(P<0.01);SOD活力显著低于普通饲料组(P<0.01);GPX活力低于普通饲料组,但无统计学差异(P>0.05). 与高血脂模型组相比,苦荞壳提取物各组大鼠肝组织Ch浓度显著降低,SOD活力显著升高(P<0.05),GPX活力升高,但无统计学差异(P>0.05);高剂量组Tg,MDA浓度显著降低(P<0.05),低剂量组Tg,MDA浓度降低,但无统计学差异(P>0.05). 力平之组大鼠肝脏Ch降低(P<0.05),Tg,MDA含量和SOD活力无显著性变化(P>0.05);而GPX活力显著降低(P<0.05). 结论: 苦荞壳提取物可减少高脂血症大鼠肝脏脂质沉积,降低肝脏脂质过氧化水平,提高肝脏抗氧化能力,预防脂肪肝的形成.

Preventive effect of tartarian buckwheat shell extract on fatty liver induced by high-lipid diet in rats

AIM: To investigate the preventive effect of tartarian buckwheat shell extract (TBSE) on fatty liver induced by high-lipid diet in rats and it mechanism. METHODS: Fifty male Wistar rats were randomly separated into 5 groups according to body weight. They were fed with normal diet, high-lipid diet, high-lipid diet supplemented with different concentrations of TBSE (0.825 g/kg, 0.165 g/kg), high-lipid diet supplemented with Lipingzhi capsules (LPZ, 0.067 g/kg), respectively. After 12 weeks, in liver tissues, Ch, Tg, MDA concentrations, SOD, GPX activities were measured, and the histological changes were observed. RESULTS: Compared with normal diet group, in high-lipid diet group liver Ch, Tg, MDA concentrations increased significantly (P<0.01); liver SOD activity decreased significantly (P<0.01); liver GPX activity decreased, but there was no significantly statistical difference (P>0.05). Compared with high lipid diet group, in TBSE groups, liver Ch concentration decreased significantly (P<0.05), liver SOD activity increased significantly (P<0.05), and liver GPX activity increased, but there was no significantly statistical difference (P>0.05); in high-dose TBSE group, liver Tg, MDA concentration decreased significantly (P<0.05); in low-dose TBSE group, liver Tg, MDA concentration decreased, but there was no significantly statistical difference (P>0.05). In LPZ group, compared with high-lipid diet group, liver Ch concentration, GPX activity decreased significantly (P<0.05); liver MDA, Tg concentration, SOD activity had no significantly statistical changes (P>0.05). CONCLUSION: TBSE could decrease liver Ch, Tg concentration, reduce liver lipid deposition and lipid peroxidation, increase liver antioxidant activity in rats with hyperlipidemia, which could protect hepatocytes from fatty degeneration.