UFLC同时测定复方氨酚烷胺片中对乙酰氨基酚和咖啡因的含量

目的：建立UFLC法测定复方氨酚烷胺片中对乙酰氨基酚和咖啡因的含量。方法：shim—packXR—ODSII C18柱（75mm×2．0mm，2．2μm），以0．058mol·L^-1的磷酸二氢铵溶液-甲醇-三乙胺（80：30：0．05pH3．4）为流动相，检测波长为215nm、柱温为40℃、流速为0．7ml·min^-1。结果：对乙酰氨基酚和咖啡因分别在0．24—6．0mg·ml^-1（r=0．9996）和0．016～0．40mg·ml^-1（r=0．9991）范围内呈现良好的线性关系；回收率分别为99．86％和99．65％，RSD分别为0．48％和0．88％（n=9）。结论：方法简便、迅速、重复性好，可作为该制剂质量控制的方法。

Simultaneous Determination of Paracetamol and Caffeine in Compound Paracetamoi and Amantadine Hydrochloride Tablets by UFLC

Objective： To establish an UFLC method for determination of paracetamol and caffeine in compound paracetamol and amantadine hydrochloride tablets. Method： Shim-pack XR-ODS II C18 column（75mm × 2.0mm,2.2μm）was used, the mobile phase was 0. 058mol. L^-1 ammonium dihydrogen phosphatemethanol -triethylamine （ 80 ： 30 ： 0.05 pH3.4 ） , the detection wavelength was 215nm. the flow rate was 0.7ml. min^-1 with the column temperatureat was 40℃. Result： The linear ranges of paracetamol and caffeine were 0.24 - 6.0mg. ml^-1, r = 0. 999 6 and 0. 016 - 0.40mg. ml^-1, r = 0. 999 1, the average recoveries were 99.86% and 99.65% with RSD 2.48% and 0.88% , respectively. Conclusion： This method is simple, rapid, and repeatable, It is available for the quality control of compound paracetamol and amantadine hydrochloride tablets.