五味消毒饮HPLC指纹图谱建立及10种成分同时测定

目的建立五味消毒饮(金银花、蒲公英、野菊花等)HPLC指纹图谱,并同时测定新绿原酸、绿原酸、隐绿原酸、秦皮乙素、咖啡酸、木犀草苷、异绿原酸B、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、蒙花苷的含量。方法该药物甲醇提取液的分析采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长320 nm。结果10批样品指纹图谱中有34个共有峰,相似度均大于0.970。10种成分在各自范围内线性关系良好(r≥0.9998),平均加样回收率95.16%~104.50%,RSD 1.09%~4.90%。结论该方法准确可靠,重复性好,可用于五味消毒饮的质量控制。

Establishment of HPLC fingerprints for Wuwei Xiaodu Drink and simultaneous determination of ten constituents

AIM To establish the HPLC fingerprints of Wuwei Xiaodu Drink(Lonicerae japonicae Flos,Taraxaci Herba,Chrysanthemi indici Flos,etc.)and to simultaneously determine the contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,glycolylic acid,caffeic acid,luteolin,iso-chlorogenic acid B,3,5-dicaffeoyl quinine,4,5-dicaffeoyl quinine and montflurin.METHODS The analysis of methanol extract of this drug was performed on a 25℃thermostatic Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 320 nm.RESULTS There were thirty-four common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.970.Ten constituents showed good linear relationships within their own ranges(r≥0.9998),whose average recoveries were 95.16%-104.50%with the RSDs of 1.09%-4.90%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Wuwei Xiaodu Drink.