荜茇根化学成分及其抗肿瘤活性

目的研究荜茇Piper longum L.根的化学成分及其抗肿瘤活性。方法荜茇干燥根茎95%乙醇提取物采用硅胶、Sephadex LH-20、重结晶进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。MTT法测定其中2种典型生物碱的抗肿瘤活性。结果从中分离得到10个化合物,分别鉴定为β-谷甾醇(1)、胡萝卜苷(2)、二十四烷酸(3)、十九烷酸(4)、荜茇酰胺(5)、胡椒碱(6)、胡椒内酰胺A(7)、胡椒内酰胺D(8)、N-异丁基-(2E,4E,12Z)-十八烷基-1-酰胺(9)、3,4-亚甲二氧基肉桂酸(10)。化合物5对3株肿瘤细胞的IC50值均小于20μmol/L。结论化合物3~4、7~10为首次从该植物中分离得到,化合物5对3株肿瘤细胞表现出显著的细胞毒活性。

Chemical constituents from the roots of Piper longum and their antitumor activities

AIM To study the chemical constituents from the roots of Piper longum L.and their antitumor activities.METHODS The 95%ethanol extract from the roots of P.longum was isolated and purified by silica,Sephadex LH-20 and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities of two typical alkaloids were determined by MTT method.RESULTS Ten compounds were isolated and identified asβ-sitosterol(1),daucosterol(2),xxivacid(3),onadecanoic acid(4),piperlongumine(5),piperine(6),piperolactam A(7),piperolactam D(8),N-isobutyl-(2E,4E,12Z)-octadecatrienamide(9),3,4-(methylenedioxy)cinnamic acid(10).The IC50values of compound 5 for three cancer cells were less than 20μmol/L.CONCLUSION Compounds 3-4,7-10 are isolated from this plant for the first time,and compound 5 shows significant cytotoxicity against three cancer cell lines.