| HPLC法测定六神丸中游离胆红素及总胆红素含量 |
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| 引用本文： | 王鑫,李俊松,狄留庆,李文,王洪兰. HPLC法测定六神丸中游离胆红素及总胆红素含量[J]. 现代中药研究与实践, 2020, 34(2): 60-64. DOI: 10.13728/j.1673-6427.2020.02.012 |
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| 作者姓名： | 王鑫 李俊松 狄留庆 李文 王洪兰 |
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| 作者单位： | 南京中医药大学附属南京中医院药剂科,江苏南京210001;南京中医药大学药学院,江苏南京210023;江苏省中药高效给药系统工程技术研究中心,江苏南京210023;南京中医药大学药学院,江苏南京210023;江苏省中药高效给药系统工程技术研究中心,江苏南京210023;南京中医药大学附属南京中医院药剂科,江苏南京210001 |
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| 基金项目： | 江苏省科技成果转化专项;南京市医学科技发展项目;国家中药标准化项目（中药标准行动计划项目） |
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| 摘 要： | 目的建立HPLC法测定六神丸中游离胆红素和总胆红素的含量。方法采用HPLC法,ODS-2色谱柱(250 mm×4.6 mm,5μm);以乙腈-1%冰醋酸水溶液(95∶5)为流动相;体积流量1 ml/min;检测波长450 nm;柱温30℃;进样量10μl。结果胆红素质量浓度在6.30~201.50μg/ml(r≥0.9991)范围内与峰面积线性关系良好;游离胆红素平均回收率为100.75%,RSD为2.25%,总胆红素平均回收率为106.66%,RSD为1.04%;六神丸中游离胆红素平均含量为0.072%,总胆红素平均含量为0.159%。结论建立HPLC法测定六神丸中游离胆红素限量检查及总胆红素的含量,此法简便快速准确,重复性好,可用于六神丸质量控制并指导临床用药。
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| 关 键 词： | 六神丸 游离胆红素 总胆红素 高效液相色谱法 |
HPLC Method for Determination of Free and Total Bilirubin in Liushen Pills |
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| WANG Xin,LI Jun-song,DI Liu-qing,LI Wen,WANG Hong-lan. HPLC Method for Determination of Free and Total Bilirubin in Liushen Pills[J]. Research and Practice on Chinese Medicines, 2020, 34(2): 60-64. DOI: 10.13728/j.1673-6427.2020.02.012 |
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| Authors: | WANG Xin LI Jun-song DI Liu-qing LI Wen WANG Hong-lan |
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| Affiliation: | (Department of Pharmacy,the Affilated Nanjing Hospital of Nanjing University of Chinese Medicine,Nanjing 210001,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Engineering Research Center for Efficient Delivery System of TCM,Nanjing 210023,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of free bilirubin and total bilirubin in Liushen pills.Methods ODS-2(250 mm×4.6 mm,5μm)column,mobile phase acetonitrile-1%glacial acetic acid solution(95∶5).Volume flow rate is 1 ml/min;Detection wavelength:450 nm;Column temperature 30℃;The injection volume is 10μl.Results The concentration of bilirubin in the range of 6.30~201.50μg/ml(r≥0.9991)has a good linear relationship with the peak area.The average recovery of free bilirubin is 100.75%,RSD is 2.25%,the average recovery of total bilirubin is 106.66%,RSD is 1.04%.The average content of free bilirubin in Liushen pills is 0.072%,and the average content of total bilirubin is 0.159%.Conclusion To establish HPLC method for determination of free bilirubin and total bilirubin in Liushen pills.This method is simple,rapid,accurate and reproducible,and can be used for quality control and clinical application of Liushen pills. |
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| Keywords: | Liushen pill free bilirubin total bilirubin HPLC |
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