基于网络药理学探讨增液汤的物质作用基础及机制研究

目的使用网络药理学探讨增液汤的物质作用基础及可能机制。方法基于BATMANTCM、TCMSP、Chemistry Database、DrugBank和UniProt公共数据平台筛选增液汤的有效化学成分及其对应靶点并建立可视化网络模型;利用David及OmicShare数据库对靶标进行KEGG通路富集、基因本体论(GO)分析;靶标相互作用分析基于STRING,并利用Cytoscape进行拓扑参数分析,寻找核心靶点及主要信号通路。结果增液汤共筛选出51个有效成分,对应靶点数目为373个;GO及KEGG富集结果显示其功能多集中在代谢、细胞增殖、免疫系统等,可能与脂肪细胞因子信号通路、钙信号通路、代谢通路等相关;靶标相互作用及网络拓扑分析可知其核心靶点共12个。结论增液汤有效成分及靶点多样,通路丰富,为其临床治疗及应用提供理论基础。

Study on the Target and Mechanism of Possible Substances in Zengye Decoction Based on Network Pharmacology

Objective To explore the material basis and possible mechanisms of Zengye Decoction based on network pharmacology.Methods Screening the effective chemical constituents of Zengye Decoction and their corresponding targets based on BATMAN-TCM,TCMSP,Chemistry Database,DrugBank and UniProt public data platforms and establishing a visual network model.Using David and OmicShare databases to analysis KEGG pathway enrichment and Gene Ontology(GO)of targets.The target interaction analysis is based on STRING and uses Cytoscape for topological parameter analysis to find core targets and major signaling pathways.Results A total of 51 active components are screened out in Zengye Decoction.The corresponding target number is 373.The results of GO and KEGG enrichment show that their functions are mostly concentrated in metabolism,cell proliferation,immune system,etc.,and may be related to adipocytokines signaling pathway,calcium signaling pathway,metabolic pathway,etc.Target interaction and network topology analysis show that its core target is 12.Conclusion The active ingredients and targets of Zengye Decoction are diverse and the pathways are multiple,providing a theoretical basis for its clinical treatment and application.