| UHPLC-PDA法同时测定毛大丁草中6种成分 |
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| 引用本文： | 游景瑞,孙佳,兰燕宇,李勇军,王永林,刘春花.UHPLC-PDA法同时测定毛大丁草中6种成分[J].中成药,2021(1):111-116. |
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| 作者姓名： | 游景瑞 孙佳 兰燕宇 李勇军 王永林 刘春花 |
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| 作者单位： | ;1.贵州医科大学民族药与中药开发应用教育部工程研究中心省部共建药用植物功效与利用国家重点实验室;2.贵州医科大学药学院;3.贵州医科大学贵州省药物制剂重点实验室 |
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| 基金项目： | 国家自然科学基金项目(82060777,U1812403);中央引导地方科技专项项目(黔科中引地[2018]4006);贵州省自然科学基金资助项目(黔科合基础[2018]1128);贵州省科学技术厅项目(黔科合平台人才[2016]5613、5677,黔科合平台人才[2017]5718);贵州省国家级、省级创新创业训练计划项目(DC 201710660006);贵州医科大学博士启动基金(YJ2017-12)。 |
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| 摘 要： | 目的建立UHPLC-PDA法同时测定毛大丁草Gerbera piloselloides(Linn.)Cass.中熊果苷、绿原酸、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C的含量。方法毛大丁草50%甲醇提取物的分析采用Waters BEH C18色谱柱(2.1 mm×150 mm,1.7μm);流动相乙腈-0.1%甲酸,梯度洗脱;体积流量0.28 mL/min;柱温40℃;检测波长280、328 nm。结果熊果苷、绿原酸、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C分别在4.45~284.59(r=0.9999)、0.30~19.52(r=0.9998)、0.30~19.50(r=0.9998)、0.31~19.84(r=0.9999)、0.61~38.96(r=0.9998)、0.32~20.19(r=0.9998)μg/mL范围内线性关系良好,平均加样回收率分别为102.06%、103.37%、104.97%、104.79%、105.21%、104.75%,RSD分别为1.88%、1.70%、1.20%、1.89%、1.53%、1.25%。结论该方法稳定,重复性好,可用于毛大丁草的质量控制。
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| 关 键 词： | 毛大丁草 化学成分 UHPLC |
Simultaneous determination of six constituents in Gerbera piloselloides by UHPLC-PDA |
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| YOU Jing-rui,SUN Jia,LAN Yan-yu,LI Yong-jun,WANG Yong-lin,LIU Chun-hua.Simultaneous determination of six constituents in Gerbera piloselloides by UHPLC-PDA[J].Chinese Traditional Patent Medicine,2021(1):111-116. |
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| Authors: | YOU Jing-rui SUN Jia LAN Yan-yu LI Yong-jun WANG Yong-lin LIU Chun-hua |
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| Institution: | (State Key Laboratory of Functions and Applications of Medicinal Plants,Engineering Research Center for Development and Application of Ethnic Medicine and TCM(Ministry of Education),Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China) |
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| Abstract: | AIM To establish a UHPLC analysis method for simultaneous determination of arbutin,chlorogenic acid,luteoloside,isochlorogenic acid B,isochlorogenic acid A and isochlorognic acid C in Gerbera piloselloides(Linn.)Cass..METHODS The analysis of 50%methanol extract of G.piloselloides was performed on a 40℃thermostatic Waters BEH C18 column(2.1 mm×150 mm,1.7μm),with the mobile phase comprising of acetonitrile and 0.1%formic acid flowing at 0.28 mL/min in a gradient elution manner,and the detection wavelengths were set at 280,328 nm.RESULTS Arbutin,chlorogenic acid,luteoloside,isochlorogenic acid B,isochlorogenic acid A and isochlorognic acid C showed good linear relationships within the ranges of 4.45-284.59(r=0.9999),0.30-19.52(r=0.9998),0.30-19.50(r=0.9998),0.31-19.84(r=0.9999),0.61-38.96(r=0.9998),0.32-20.19(r=0.9998)μg/mL,whose average recoveries were 102.06%,103.37%,104.97%,104.79%,105.21%,104.75%with the RSDs of 1.88%,1.70%,1.20%,1.89%,1.53%,1.25%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.piloselloides. |
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| Keywords: | Gerbera piloselloides(Linn )Cass chemical constituents UHPLC |
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