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| RP-HPLC法测定菌毒清颗粒中哈巴俄苷的含量 | |
| 引用本文： | 宋粉云,张俊芳,钟兆健,肖若媚. RP-HPLC法测定菌毒清颗粒中哈巴俄苷的含量[J]. 中国药房, 2007, 18(24): 1874-1875 |
| 作者姓名： | 宋粉云 张俊芳 钟兆健 肖若媚 |
| 作者单位： | 1. 广东药学院,广州市,510224 2. 广东省人民医院药学部,广州市,510080 3. 广东中山市人民医院,中山市,528402 |
| 摘 要： | 目的:建立以RP-HPLC法测定菌毒清颗粒中哈巴俄苷含量的方法。方法:色谱柱为DiamonsidTM-C18(250mm×4.6mm,5μm),流动相为乙腈-1%醋酸溶液(二元梯度洗脱),流速为1.0mL.min-1,检测波长为278nm,柱温为室温。结果:哈巴俄苷检测浓度在2.55～20.4μg.mL-1范围内与峰面积积分值呈良好线性关系;平均回收率为96.6%,RSD=0.67%(n=6)。结论:本法简便、准确,可用于菌毒清颗粒的质量控制。 |
| 关 键 词： | 菌毒清颗粒 哈巴俄苷 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408（2007）24-1874-02 |
| 收稿时间： | 2007-01-14 |
| 修稿时间： | 2007-01-142007-05-17 |
Determination of Harpagosicle in Junduqing Granule by RP- HPLC Method |
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| SONG Fenyun,ZHANG Junfang,ZHONG Zhaojian,XIAO Ruomei. Determination of Harpagosicle in Junduqing Granule by RP- HPLC Method[J]. China Pharmacy, 2007, 18(24): 1874-1875 | |
| Authors: | SONG Fenyun ZHANG Junfang ZHONG Zhaojian XIAO Ruomei |
| Abstract: | OBJECTIVE:To develop a RP-HPLC method for the determination of Harpagosicle in Junduqing granule.METHODS:The chromatographic column was Diamonsil-C18(250mm× 4.6mm,5μ m) at room temperature.The mobile phase was acetonitrile-1% HAc(gradient elution).The flow rate was 1.0mL· min-1 and the detection wavelength was 278 nm.RESULTS:The linear range of Harpagosicle was 2.55～ 20.4μg· mL-1,with average recovery at 96.6%,RSD=0.67%(n=6).CONCLUSION:The method was proved to be sensitive and accurate,and it can be used as quality control of Junduqing granule. |
| Keywords: | Junduqing granule Harpagosicle RP-HPLC Content determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |