HIV抗体筛查阳性标本确证结果分析

目的通过对2 344例HIV抗体筛查阳性标本确证实验结果的分析,探讨筛查试验结果与确证试验结果之间的关系。方法筛查和复检:ELISA和快速检测;确证:免疫印迹(WB)法。结果筛查阳性与确证阳性总体符合率较高(86.22%),且逐年有所提高;无偿献血人群符合率较低(33.33%),不确定结果多为非特异性反应。结论确定HIV感染必须进行WB确证试验以便排除假阳性反应,无偿献血人群筛查假阳性较高,应慎重诊断。

Analysis on Confirmatory Test Results of Positive Samples of HIV Antibody Screening

Objective To explore the relationship between screening test results and confirmatory test results through analyzing 2,344 positive samples of HIV antibody screening.Methods ELISA was used for HIV antibody screening.Rapid immunochromatographic assay was applied in re-examination.Western blotting was adopted in confirmatory test.Results The total coincidence rate of screening positive samples and confirming positive samples was higher（86.22%）.Moreover,it was increased year by year.The coincidence rate in voluntary blood donors was lower（33.33%）,and most indeterminate results were attributed to non-specific reactions.Conclusions Western blotting is needed to confirm the infection of HIV and exclude false-positive reaction.The false-positive reactions in voluntary blood donors are higher,which should be diagnosed carefully.