| 大鼠听皮质NMDA受体亚单位NR2B蛋白质的年龄-依赖性表达 |
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| 引用本文： | 张凌,吴秀梅,徐凤,许兢宏,孙心德.大鼠听皮质NMDA受体亚单位NR2B蛋白质的年龄-依赖性表达[J].解剖学杂志,2005,28(5):566-569. |
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| 作者姓名： | 张凌 吴秀梅 徐凤 许兢宏 孙心德 |
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| 作者单位： | 华东师范大学生命科学学院脑科学研究中心,上海200062 |
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| 基金项目： | 国家自然科学基金（30170313）.博士点基金（20020269005）.上海市神经科学重点学科项目 |
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| 摘 要： | 目的：研究sD大鼠生后发育过程中，听皮质神经元NMDA受体亚单位NR2B蛋白质的表达规律。方法：采用免疫组织化学反应和蛋白质印迹技术，分别检测生后1、2、3周和成年动物听皮质神经元NMDA受体亚单位NR2B蛋白质的表达。结果：NR2B阳性神经元在生后第1周密度最高，随着生后周龄增长，NR2B阳性神经元密度递减．3周龄后降至成年动物的低表达水平。结论：大鼠生后发育过程巾．听皮质NR2B亚单位蛋白质呈现年龄-依赖性表达，此结果与mRNA水平上的表达趋势一致。
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| 关 键 词： | 大鼠 听皮质 免疫组织化学 NMDA受体 NR2B亚单位 |
Age-dependent expression of NMDA receptor subunit NR2B protein in rat auditory cortex |
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| ZHANG Ling, WU Xiu Mei, XU Feng, XU Jing Hong, SUN Xin-De.Age-dependent expression of NMDA receptor subunit NR2B protein in rat auditory cortex[J].Chinese Journal of Anatomy,2005,28(5):566-569. |
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| Authors: | ZHANG Ling WU Xiu Mei XU Feng XU Jing Hong SUN Xin-De |
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| Institution: | College o f Life Science, Research Center ,for Brain Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | Objective: To study the age-dependent expression of NMDA receptor subunit NR2B protein during postnatal development in the rat auditory cortex.Methods: Western-blotting and immunocytochemistry techniques were used to examine NR2B protein expression on postnatal week(PNW) 1,2,3 and in adult rats.Results: The density of NR2B-positive neurons was detected in the auditory cortex during development.The protein expression of NR2B was the highest on PNW 1,and then began to decline by PNW 3 to a low level as in adults.Conclusion: Age-dependent expression of NR2B protein in rats during postnatal period is consistant with the mRNA levels,which may help to understand the molecular mechanism of developmental plasticity of auditory cortex. |
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| Keywords: | rat auditory cortex immunohistochemistry NMDA receptor subunit NR2B |
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