急性白血病系列特异性抗原表达分析

Objective To investigate the differential expression of serial specific antigens in patients with acute leukemia(AL)and its clinical significance.Methods Multi-parameter flow cytometry and CD45/SSC gating were used to analyze the surface and cytoplasmic antigen expression in juvenile cells of 183 AL patients.Results CD13,CD33 and CD117 were the most commonly expressed surface antigens in acute myeloid leukemia(AML)patients(95.0%,95.7%and 81.4% respectively).The specificity of CD117 was higher than that of CD13 and CD33(P＜0.01).There were no significant differents in sensitivity and specificity between cytoplasmic antigen MPO and surface antigen CD117(P＞0.05).In B-lineage acute lymphocytic leukemia(ALL),the sensitivity of CD19 was higher than CD20,but the specificity was similar tO CD20.cCD79a showed high sensitivity and specificity.In T-lineage ALL,the sensitivity of CD7 was high(95.0%),but the specificity was low(70.8%).But the sensitivity and specificity of cCD3 was 85.0% and 95.7% respectively.Conclusion Muli-parameter flow cytometry is a reliable technique in monitoring the expression of lineage-related surface/cytoplasmic antigens in the diagnosis of acute leukemia.

Expression of serial specific antigens in patients with acute leukemia