白芷香豆素类化合物的成分分析及CO_2超临界萃取工艺的研究

目的:建立以高效液相色谱(HPLC)法分析白芷香豆素类化合物的成分及CO2超临界流体法萃取白芷中香豆素类成分的的方法。方法:采用正交试验设计考察萃取压力、萃取温度、萃取时间及药材粒度对总香豆素收率的影响,用HPLC法检测萃取物中欧前胡素和异欧前胡素的含量。结果:欧前胡素、异欧前胡素的检测浓度线性范围分别为29.4～235.2、10～80μg/ml,平均回收率分别为(100.46±1.42)%、(99.94±1.18)%(n=3),相对标准差分别为1.76%、1.62%;以无水乙醇作为改性剂,白芷在萃取压力35MPa、萃取温度45℃、药材粒度60目、萃取时间3h时总香豆素收率最高。结论:萃取压力、药材粒度、萃取时间对总香豆素萃取收率均有显著影响;所建立的分析方法精密度高、简便快速,适于欧前胡素和异欧前胡素的含量测定。

Analysis of Coumarins from Angelica Dahurica and CO2 Supercritical Fluid Extraction Method for Extraction of Coumarins

OBJECTIVE:To establish a HPLC method for determination of total coumarins in Angelica dahurica and to develop a CO 2 supercritical fluid extraction(SFE)method for its extraction.METHODS:Orthogonal design was used to inˉvestigate the influence of extraction pressure,temperature,time length and pulverized degree of medicine on extraction rate of total coumarins.The contents of imperator and isoimperator in the extract were analyzed by HPLC.RESULTS:The Liner ranges of imperator and isoimperator were29.4～235.2?10～80μg/ml respectively.The average recoveries were(100.46±1.42)%?(99.94±1.18)%(n=3)respectively.The relative standard deviations were1.76%and1.62%respectively.When alcohol was used as a modifier,the extraction rate of total coumarins was the highest when the sample was pulverized into60mesh and had been extracted at35MPa and45℃for3hours.CONCLUSIONS:The extraction pressure,pulverized degree and extraction length all have significant effects on extraction rate of total coumarins.The established method is precise,convenient and can be used for determination of imperator and isoimperator.