Bone marrow mesenchymal stem cells can be mobilized into peripheral blood by G-CSF in vivo and integrate into traumatically injured cerebral tissue |
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Authors: | Jun Deng Zhong-min Zou Tao-li Zhou Yong-ping Su Guo-ping Ai Jun-ping Wang Hui Xu Shi-wu Dong |
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Institution: | (1) Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, School of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, Chongqing, 400038, China;(2) Department of Pathogenic Biology, School of Medical Science, The Third Military Medical University, Chongqing, 400038, China; |
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Abstract: | The efficacy of granulocyte colony-stimulating factor (G-CSF) in mobilizing mesenchymal stem cells (MSCs) into peripheral
blood (PB) and the ability of PB-MSCs incorporated into injured brain were tested. Colony forming, cell phenotype and differentiation
potential of mouse MSCs mobilized by G-CSF (40 μg/kg) were evaluated. Mortality and pathological changes in mice with serious
craniocerebral trauma plus G-CSF treatment (40 μg/kg) were investigated. Bone marrow (BM) cells derived from GFP mice were
fractionated into MSCs, hematopoietic stem cells (HSCs), and non-MSC/HSCs using magnetic beads and adherent culture. The resultant
cell populations were transplanted into injured mice. The in vivo integration and differentiation of the transplanted cells
were detected immunocytochemically. The expression of SDF-1 in injured area of brain was tested by Western blot. G-CSF was
able to mobilize MSCs into PB (fourfold increase). PB-MSCs possessed similar characteristics as BM-MSCs in terms of colony
formation, the expression pattern of CD73, 44, 90, 106, 31 and 45, and multipotential of differentiation. Accumulative total
mice mortality was lower in TG group (5/14) than that in T group (7/14). It was MSCs, not HSCs or non-MSC/HSC cells integrated
into the damaged cerebral tissue and differentiated into cells expressing neural markers. Increased SDF-1 expression in injured
area of brain was confirmed, which could facilitate the homing of MSCs to brain. G-CSF can mobilize MSCs into PB and MSCs
in PB can integrate into injured cerebral tissue and transdifferentiated into neural cells and may benefit the repair of trauma. |
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