高尿酸血症对大鼠血压和血清脂联素的影响

目的观察高尿酸血症对大鼠血压和血清脂联素的影响,探讨高尿酸血症导致的血清脂联素的变化与血管内皮功能的关系。方法 36只雄性SD大鼠随机分为3组。使用高酵母膏饲料联合氧嗪酸钾悬液腹腔注射6周诱导大鼠高尿酸血症(n=12)。别嘌醇组(n=12)在给予酵母提取物和氧嗪酸钾同时给予别嘌醇灌胃。普通饲料饲养的大鼠作为对照(n=12)。测量大鼠收缩压。6周后处死大鼠,全自动生化分析仪检测血清尿酸,ELISA法检测血清脂联素、一氧化氮和内皮素1,免疫组织化学法检测大鼠主动脉内膜层内皮型一氧化氮合酶的表达量。结果与正常对照组相比,模型组大鼠血尿酸、内皮素1、收缩压显著升高,血清脂联素、一氧化氮及主动内膜层内皮型一氧化氮合酶表达量显著降低。与模型组相比,别嘌醇组血尿酸、内皮素1、收缩压降低,血清脂联素和一氧化氮水平升高,主动脉内膜内皮型一氧化氮合酶表达增加。脂联素与血尿酸、内皮素1、收缩压呈负相关。结论高尿酸血症诱导的大鼠高血压与血清脂联素、一氧化氮的降低及内皮素的升高有关。

The Influence of Hyperuricemia on Blood Pressure and Serum Adiponectin in Rats

Aim To investigate the impact of hyperuricemia(HUA) on blood pressure and serum adiponectin(APN) and to explore the relationship between endothelial function and the change of serum APN induced by hyperuricemia. Methods Thirty six male Sprague-Dawley(SD) rats were randomly divided into 3 goups.HUA was induced in rats over 6 weeks by a high-yeast extract(YE) diet united intraperitoneal injection of potassium oxonate(OA) suspension(n=12).Allopurinol was administered simultaneously with the OA and YE in allopu...