早期梅毒螺旋体感染最适检测方法的探究

目的：探究早期梅毒螺旋体感染的最适检测方法。方法：采用实时荧光定量聚合酶链反应（FQ-PCR）、梅毒螺旋体血凝试验（TPHA）、梅毒螺旋体酶联免疫吸附试验（TP-ELISA）、快速血清反应（RPR）法对102例早期疑似梅毒患者、30例非梅毒患者分泌物、血清进行梅毒螺旋体（TP）检测,比较4种检测法灵敏度及特异性。结果：102例早期疑似梅毒患者FQ-PCR检出92例检出率90.20%,TPHA检出86例检出率84.31%,TP-ELISA检出88例检出率86.27%,RPR检出70例检出率68.6%。30例非梅毒患者FQ-PCR检测全阴性,TPHA检出2例阳性,TP-ELISA检出1例,RPR检出6例阳性。结论：早期梅毒患者诊断首选FQ-PCR进行分泌物或溃疡创面渗出物检测TP-DNA,其次选用TPHA和TP-ELISA进行血清学检测,RPR在梅毒感染早期检出率较低,易出现漏检现象。

Investigation of early syphilis infection

Objective:To investigate early syphilis infection.Methods:A total of 102 suspected cases of early syphilis patients and 30 patients with non-secretory syphilis were detected by real-time quantitative polymerase chain reaction(FQ-PCR),treponema pallidum hemagglutination test(TPHA),treponema pallidum enzyme-linked immunosorbent assay(TP-ELISA) serum rapid reaction(RPR) and serum treponema pallidum(TP) detection.Comparing the four detection sensitivity and specificity.Results:102 cases of early syphilis in patients with suspected FQ-PCR Detection of 92 cases detectable rate of 90.20%,TPHA detectable 86 cases detectable rate 84.31%.TP-88 is detectable by ELISA and detection rate of 86.27%,RPR detectable 70 cases detectable rate 68.6%.30 cases of syphilis patients with non-FQ-PCR all negative,TPHA detectable two cases positive,TP-1 is detectable by ELISA,RPR detectable positive six cases.Conclusion:Early diagnosis of syphilis patients preferred FQ-PCR secretions positive six cases.Conclusion:Early diagnosis of syphilis patients preferred FQ-PCR secretions or ulcer leakage detection TP-DNA,Second choice TPHA and TP-ELISA for serological testing,RPR in the early detection of syphilis infection rate lower,easily missed phenomenon.