白芍总苷对腹膜纤维化大鼠HMGB-1、Smad7的影响

目的:观察白芍总苷对腹膜纤维化大鼠HMGB-1、Smad7蛋白表达的影响。方法:选用健康SD大鼠32只,随机分为空白组、模型组、白芍总苷高剂量组、白芍总苷低剂量组,每组8只。用4.25%腹膜透析液每日腹腔注射和脂多糖间断腹腔注射进行大鼠腹膜纤维化造模。白芍总苷高剂量组每日给予白芍总苷200 mg/(kg·d)灌胃,白芍总苷低剂量组每日给予白芍总苷100 mg/(kg·d)灌胃;实验第30天结束后酶联免疫分析(ELISA)检测大鼠透析液中HMGB-1、Smad7的蛋白质表达情况。结果:模型组、白芍总苷高剂量组、白芍总苷低剂量组腹透液中HMGB-1水平与空白组相比有所增高,白芍总苷两组与模型组相比较,HMGB-1水平有所下降,但各组间差异无统计学意义(P>0.05)。模型组与空白组比较,腹透液中Smad7的含量显著升高,差异有统计学意义(P<0.05)。白芍总苷两组升高更为明显,与空白组相比,差异有统计学意义(P<0.01);与模型组相比较,白芍总苷两组腹透液中Smad7水平明显上调,差异有统计学意义(P<0.05)。余各组间两两比较,腹透液中Smad7含量无统计学差异(P>0.05)。结论:白芍总苷可一定程度下调HMGB-1水平,但作用机理不确切,可能通过上调Smad7的表达来减轻大鼠的腹膜纤维化。

The Effect of Total Glucosides of Paeony on Membrane Permeability on HMGB-1 and Smad7 in Peritoneal Fibrosis Rats

Objective： To explore the effect of the total glucosides of paeony（TGP） on HMGB-1 and Smad7 on membrane permeability in peritoneal fibrosis rats. Methods： Thirty-two healthy SD male rats were equally randomized into 4 groups： the control group, the model group, the TGP high-dose and low-dose group. Rat model of peritoneal fibrosis was induced by high glucose dialysate（4.25%, Baxter） and lipopoly saccharide（LPS）. The rats in the TGP high-dose group were given TGP 200 mg/（kg·d）, while in the TGP low-dose group were given TGP 100 mg/（kg·d）, both of the two groups were given by gastric gavage in the morning once a day. Thirty days later, HMGB-1 and Smad7 in dialysate were detected by ELISA method. Results： Compared with the control group, the HMGB-1levels of dialysate in the model group, TGP high dose group and TGP low dose group, had increased; compared with the model group, the HMGB-1 levels in TGP high dose group and TGP low dose group had decreased, but the differences among the groups were not statistically significant（P〉0.05）. Compared with the control group, the Smad7 levels of dialysate in the model group was significantly increased, and the difference was statistically significant（P〈0.05）. Compared with the control group, TGP high dose group and TGP low dose group had increased more significantly, the difference were statistically significant（P〈0.01）; compared with the model group, the Smad7 levels of dialysate in TGP high dose group and TGP low dose group were significantly increased, the difference were statistically significant（P〈0.05）. Between the TGP two groups, the difference were not statistically significant（P〉0.05）. Conclusion： TGP can do a certain extent to cut HMGB-1 level, but the mechanization is not exact. Maybe TGP do its work by increasing the expression of Smad7 to reduce rat peritoneal fibrosis.