微透析法进行盐酸青藤碱大鼠体外血浆蛋白结合率 的测定及与超滤法的比较

目的:考察微透析技术进行蛋白结合率研究的可行性.方法:以微透析技术为采样手段,以HPLV-UV为检测工具,采用零净通量法测定了高、中、低浓度下盐酸青藤碱的大鼠离体血浆蛋白结合率,同时采用超滤法测其蛋白结合率,比较二者结果的异同.结果:微透析法测定的盐酸青藤碱大鼠蛋白结合率在所研究的浓度范围内保持了相对稳定,约为26%,与超滤法测定结果无显著区别.结论:盐酸青藤碱的蛋白结合率较低,微透析技术是可行的新型蛋白结合率研究方法.

Rat in vitro plasma-binding percentage determination of sinomenine hydrochloride by using microdialysis method and ultrafiltration

Objective : To assess feasibility of plasma protein-binding percentage by using microdialysis method. Method : Sinomenine hydrochloride was selected as model drug. The in vitro rat plasma protein-binding percentage of three kinds of concentration was determinated by using microdialysis sampling tool and HPLC-UV. At the same time the classical study method, such as ultrafiltration metod, was also used to determinate the protein-binding percentage to compare the difference between the two methods. Result : The in vitro rat plasma protein-binding percentage of sinomenine hydrochloride using microdialysis method was about 26% and kept relative stable at the concentration range. There was no significant difference between the two methods. Conclusion : The results shows that the binding percentage is relative low. Microdialysis method is a new method for the study of protein-binding degree.