苦参方凝胶离体透皮吸收研究

目的：制备治疗过敏性皮肤病的传统中药方剂苦参方提取物凝胶,并考察其离体透皮吸收效果。方法：苦参方有效部位生物碱的提取采取醇提-大孔树脂纯化法,挥发油提取采用水蒸气蒸馏法。将提取物制成凝胶,以离体SD大鼠腹部皮肤为渗透屏障,采用Valia-Chien水平扩散池和Franz垂直扩散池,比较苦参方水提液、苦参方纯化提取物溶液、苦参方凝胶的透皮吸收效果,HPLC测定透皮接收液中苦参生物碱的含量。结果：以累积透皮量、透皮速率、时滞为指标,苦参方纯化提取物生物碱的透皮吸收效果优于水提物,制成凝胶后,透皮吸收效率显著改善。结论：苦参方凝胶可以显著改善苦参生物碱的透皮吸收效果,是适合局部治疗过敏性炎症的剂型。

Studies on cutaneous permeation in vitro of Kushen recipe gel

Objective:To establish a suitable dosage form for a traditional anti-anaphylaxis Chinese medicine of Kushen reci- pe,and investigate the effect of cutaneous permeation in vitro of the recipe.Method:Techniques of extracting with ethanol and purif- ying with absorbent resin to obtain alkaloids from Kushen recipe were adopted,while volatile oil was extracted by steam distillation. The extraction was made to gel.The skin from SD rats' abdomen was used as permeability barriers.Then effects of permeation of the aqueous extrcation,the purifying extraction and the gel were compared by Valia-Chien and Franz diffusion cell method.HPLC was uti- lized to quantitate the alkaloids in permeating liquid.Result:In view of the permeation cumulation quantity,the permeation velocity and the lag time of the four kinds of alkaloids,the effect of permeation of purifying extraction was better than the aqueous extrcation, and the purifying extraction gel surpassed both the aqueous extrcation and the purifying extraction.Conclusion:It was certified that the purifying extraction gel had improved the effect of cutaneous permeation of alkaloids,and it is the befitting dosage form for Kushen reci- pe to treat anaphylaxis disease in skin.