脂肪干细胞与Bio-oss支架构建组织工程骨治疗种植体周围骨缺损

背景：目前治疗种植体周围炎的方法主要有手术治疗、激光治疗、超声洁治加局部用药单独或联合治疗等，但并未完全达到理想的治疗效果。 目的：探讨由骨形态发生蛋白修饰的脂肪干细胞与Bio-oss支架构建组织工程骨治疗种植体周围炎性骨缺损的可行性。 方法：应用计算机检索1988年1月至2011年12月Pubmed数据库相关文章，检索词为“adipose-derived stem cells、tissue engineering”，并限定文章语言种类为英文。同时计算机检索2005年1月至2011年12月CNKI数据库相关文章，检索词为“脂肪干细胞、种植体周围炎”，并限定文章语言种类为中文。共检索到文献56篇。 结果与结论：利用组织工程原理与方法再生组织的特点和优势，将脂肪干细胞作为理想的种子细胞，骨形态发生蛋白2作为理想的细胞因子与Bio-oss生物材料混合，构成三维环境，Bio-oss晶体内广泛交织的空隙结构，有利于脂肪干细胞的迁移。骨形态发生蛋白2与脂肪干细胞复合，促进控制其分化向骨形成中心募集，并分化为骨系细胞，通过钙盐沉积形成新骨，达到治疗种植体周围骨缺损的目的。

Reconstruction of tissue-engineered bone using adipose-derived stem cells and Bio-oss scaffold for the treatment of peri-implantitis

BACKGROUND:Current treatments of peri-implantitis include surgery, laser therapy, ultrasonic scaling, local medication or their combination. However, there have been no ideal therapeutic effects achieved. OBJECTIVE:To investigate the feasibility of treating peri-implantitis using tissue-engineered bone reconstructed by bone morphogenetic protein-modified adipose-derived stem cells and Bio-oss scaffold. METHODS:A computer-based online retrieval of Pubmed database was performed to search papers published during January 1988 to December 2011 using the key words “adipose-derived stem cells, tissue engineering” in English. Simultaneously, related papers published during January 2005 to December 2011 in CNKI were retrieved using the key words “adipose-derived stem cells, tissue engineering” in Chinese. A total of 56 papers were retrieved. RESULTS AND CONCLUSION:According to the characteristics and advantages of regenerative tissues in tissue engineering, a three-dimensional environment was established using adipose-derived stem cells as ideal seed cells, bone morphogenetic protein 2 as an ideal cytokine and Bio-oss biomaterial. The multiporous structure of Bio-oss biomaterial facilitates the migration of adipose-derived stem cells. After compounded by adipose-derived stem cells, bone morphogenetic protein 2 promotes osteogenic differentiation of adipose-derived stem cells, leading to new bone formation by deposition of calcium salt and achieving the purpose of treating peri-implantitis.