四种消毒方式对简易呼吸囊的灭菌效果评价

目的研究观察4种不同消毒方式对简易呼吸囊的消毒效果和微生物残留状况，探讨加强呼吸囊消毒方式方法管理的必要
性。方法分4组消毒试验：G1组43例，500 mg/L二氧化氯（ClO2）喷雾；G2组28例，酒精喷雾；G3组47例，50 mg/L三氯异氰尿
酸（TCCA）浸泡；G4组46例，50 mg/L ClO2溶液浸泡。各组于消毒后30 min取样接种培养，菌落计数后用卡方秩和进行统计学
分析，并对残留菌鉴定分型。结果（1）4 组菌落计数有显著性差异（P<0.001）。细菌残留率：G1 组为0%，G2 组53.6%，G3 组
27.7%，G4组21.7%，各组间比较统计学有显著性差异（P<0.001）。（2）残留菌鉴定结果显示铜绿假单胞杆菌和饱曼不动杆菌，溶
血性葡萄球菌等常见耐药性致病菌。结论麻醉简易呼吸囊以500 mg/L ClO2喷雾方式消毒消果最好。中低效TCCA反复浸泡
长期使用，可能导致耐药致病菌生长。

Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles

Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial
residue. Methods The disinfection tests were divided into 4 groups: G1 group (43 cases) with 500 mg/L chlorine dioxide
spray, G2 group (28 cases) with alcohol spray, G3 group (47 cases) with 50 mg/L trichloro isocyanuric acid (TCCA) immersion,
and G4 group (46 cases) with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was
examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods
showed significant differences in bacterial colony count (P<0.001). The rate of bacterial residue was 0% in G1 group, 53.6% in
G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups (P<0.001). The
residual bacteria included antibiotic-resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter
baumannii and Staphylococcus haemolytic. Conclusions Disinfection with 500 mg/L chlorine dioxide spray is the best for simple
breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.