正畸力作用下大鼠牙周组织白细胞介素6 mRNA的表达

背景：白细胞介素6作为一种重要的细胞因子,在炎症部位调节了免疫反应并可通过自分泌和旁分泌的方式刺激破骨细胞的形成和骨吸收,与正畸牙移动骨改建相关。目的：观察正畸力对大鼠牙周组织中白细胞介素6 mRNA表达的影响。方法：建立大鼠正畸牙移动模型并运用原位杂交法观测加力后1,3,5,7,10,14 d白细胞介素6 mRNA在各组大鼠牙周组织内的表达情况。结果与结论：正常大鼠牙周组织内白细胞介素6 mRNA呈弱阳性表达;加力组大鼠牙周组织内白细胞介素6mRNA的表达在加力后3 d达到高峰,之后开始下降。实验提示正畸力虽可引起局部牙周组织白细胞介素6mRNA的表达增强,但具有一定的自限性。白细胞介素6作为多功能细胞因子,在正畸牙移动牙周改建中起重要作用。中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程全文链接：

Orthodontic force effects on the expression of periodontal interleukin-6 mRNA in rats

BACKGROUND: As an important cytokine, interleukin-6 regulates immune responses in inflammation sites and has an autocrine/paracrine activity that stimulates osteoclast formation and bone resorption, which is related to bone remodeling during orthodontic tooth movement.OBJECTIVE: To investigate the effects of orthodontic force on the expression of interleukin-6 mRNA in the periodontal tissue of rats. METHODS: In situ hybridization was performed to measure the expression of interleukin-6 mRNA at 1, 3, 5, 7, 10 and 14 days after the application of orthodontic force on the maxillary first molars of rats.RESULTS AND CONCLUSION: The expression of interleukin-6 mRNA was observed at a low level in the normal periodontal tissue of rats. After the application of force, the induction of interleukin-6 mRNA was observed to reach a maximum on day 3 and to decline thereafter. The expression of interleukin-6 mRNA can be evoked by orthodontic force but with a certain self-limiting. As a multifunctional cytokine, interleukin-6 plays a very important role in periodontal remodeling during orthodontic tooth movement.