超脉冲CO2点阵激光干预兔耳浅表性瘢痕的原位再生

背景：临床上超脉冲CO2点阵激光治疗浅表性瘢痕的效果优良，但机制不清。 目的：进一步验证超脉冲CO2点阵激光干预兔耳浅表性瘢痕的效果，并探讨创面原位再生修复的相关机制。 方法：用超脉冲CO2点阵激光干预兔耳浅表性瘢痕，传统络合碘治疗和湿润烧伤膏治疗两种修复方法对创面进行修复。 结果与结论：湿润烧伤膏组创面愈合显著短于络合碘组(P < 0.01)。超脉冲CO2点阵激光干预兔耳浅表性瘢痕后即刻创缘无表皮干细胞标志分子细胞角蛋白19、P63蛋白表达，24 h后细胞角蛋白19、P63蛋白表达。湿润烧伤膏组治疗后第4天细胞角蛋白19、P63蛋白开始增多，第7天达到高峰，第14天逐渐减少；络合碘组治疗后第4天细胞角蛋白19、P63蛋白达高峰，第7天和第14天减少。湿润烧伤膏组细胞角蛋白19、P63蛋白强阳性表达率显著高于络合碘组(P < 0.05)。提示超脉冲CO2点阵激光结合湿润暴露疗法治疗兔耳浅表性瘢痕，能更好地启动和保护表皮干细胞，促进创面原位再生修复。

Ultrapulse CO2 fractional laser intervens in situ superficial scar regeneration in a rabbit ear

BACKGROUND:The ultrapulse CO2 fractional laser has a good effect on the treatment of superficial scars, but the mechanism is unclear. OBJECTIVE:To investigate the effect of ultrapulse CO2 fractional laser on the treatment of superficial scars in rabbit ear and to explore mechanism of wound in situ regeneration and repair. METHODS:The superficial scars in rabbit ear were induced with ultrapulse CO2 fractional laser, then traditional iodophor treatment and moist exposed burn ointment treatment methods were used for repairing the wound. RESULTS AND CONCLUSION:The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P < 0.01). The epidermal stem cells emerged after the superficial scars induced with ultrapulse CO2 fractional laser, and the expression of cytokeratin 19 and P63 protein was observed after induced for 24 hours. The number of cytokeratin 19 and P63 protein in the moist exposed burn ointment was increased after induced for 4 days and reached peak at 7 days and then decreased at 14 days. While the number of cytokeratin 19 and P63 protein in the iodophor group was increased after induced for 4 days, and then decreased after induced for 7 and 14 days. The expression of cytokeratin 19 and P63 protein in the moist exposed burn ointment group was significantly higher than that in the iodophor group (P < 0.05). Ultrapulse CO2 fractional laser combined with moist exposed burn ointment for the treatment of superficial scar in rabbit ear can better activate and protect the epidermal stem cells, and promote wound repair and in situ regeneration.