可塑形脱矿骨基质/透明质酸骨泥的制备与细胞相容性 |
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作者姓名: | 张乃丽 张育敏 周 沫 杨 婷 王旭昇 马绍英 徐伟俊 董 丽 李宝兴 |
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作者单位: | 中国辐射防护研究院,山西省医用组织库,山西省太原市 030006 |
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基金项目: | 中国辐射防护研究院青年科技基金资助项目(YQ120807)* |
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摘 要: | 背景:应用预制成形的材料修复不规则骨缺损时,常出现材料与骨缺损形状不匹配的问题,制备可任意塑形骨泥对促进不规则骨缺损的愈合具有重要意义。
目的:构建可塑形脱钙骨基质/透明质酸骨泥,筛选最佳复合比例并评价其细胞相容性。
方法:选取合格供体皮质骨制备脱钙骨基质。分别配置浓度为0.75%,1.5%,3%,4.5%的透明质酸溶液并检测其黏度,以450 mg脱钙骨基质与上述各浓度透明质酸溶液1 mL混合,制备不同比例的脱钙骨基质/透明质酸骨泥。37 ℃条件下将骨泥浸泡在PBS平衡液中,观察其离散时间,筛选最佳复合比例的脱钙骨基质/透明质酸骨泥。应用脱钙骨基质/透明质酸骨泥浸提液培养L-929小鼠成纤维细胞,1,4,7 d采用CCK-8法检测骨泥的细胞毒性。
结果与结论:随透明质酸浓度的增加其溶液黏度增大,脱钙骨基质/透明质酸骨泥的离散时间延长,3%浓度的透明质酸与450 mg脱钙骨基质复合制备的骨泥具有良好可塑形能力,离散时间为8 h,为最佳复合比例。脱钙骨基质/透明质酸骨泥浸提液培养L-929小鼠成纤维细胞1,4,7 d的细胞增殖率分别为93.72%,101.65%,97.68%,细胞毒性级别为0或1级。表明脱钙骨基质/透明质酸复合质量比为15∶1制备的骨泥具有良好的可塑形能力及抗离散能力,细胞毒性轻微。
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关 键 词: | 生物材料 材料生物相容性 骨组织构建 可塑形骨泥 脱钙骨基质 透明质酸 黏度 抗离散能力 细胞相容性 骨修复材料 其他基金 |
Preparation and cell compatibility of malleable demineralized bone matrix/hyaluronic acid putty |
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Authors: | Zhang Nai-li Zhang Yu-min Zhou Mo Yang Ting Wang Xu-sheng Ma Shao-ying Xu Wei-jun Dong Li Li Bao-xing |
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Institution: | Medical Tissue Bank, China Institute for Radiation Protection, Taiyuan 030006, Shanxi Province, China |
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Abstract: | BACKGROUND:It is important to prepare a malleable putty to repair irregular bone defects because the preformed bone substitute material cannot suit to bone defects.
OBJECTIVE:To prepare malleable demineralized bone matrix/hyaluronic acid putty and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.
METHODS:Demineralized bone matrix was prepared from the cortical bone of healthy donors. The hyaluronic acid was dissolved at concentrations of 0.75%, 1.5%, 3%, 4.5%, and the viscosity of them was measured. Then 450 mg demineralized bone matrix was compounded with 1 mL hyaluronic acid solution to prepare the demineralized bone matrix/hyaluronic acid putty, and the optimal ratio of demineralized bone matrix/hyaluronic acid was screened by the disintegrated time of the putty at 37 ℃. L-929 mouse fibroblasts were cultured in the leaching liquor of demineralized bone matrix/hyaluronic acid, and the cytotoxicity was tested by cell count kit-8 assay at days 1, 4 and 7.
RESULTS AND CONCLUSION:With the increase of hyaluronic acid concentration, the viscosity of hyaluronic acid solution was increased; the demineralized bone matrix/hyaluronic acid putty was strengthened and the disintegrated time was prolonged gradually. When the ratio of demineralized bone matrix/hyaluronic acid was 15/1, demineralized bone matrix/hyaluronic acid was molded easily, and the disintegrated time was 8 hours in 7 ℃ PBS. The results of cytotoxicity showed that cell proliferation rates were 93.72%, 101.65% and 97.68% at days 1, 4 and 7 respectively, while the cytotoxicity was in grade 0 or 1. These findings indicate that demineralized bone matrix/hyaluronic acid putty at a compound ratio of 15:1 can be molded easily and difficult to disintegrate, with low cytotoxicity. |
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Keywords: | biocompatible materials bone matrix hyaluronic acid histocompatibility testing |
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