纳米TiO2:C薄膜涂层的生物相容性评价

背景：前期实验采用溶胶-凝胶法在纯钛片表面制备了纳米TiO₂:C薄膜涂层。 目的：评估纳米TiO₂:C薄膜涂层的生物相容性。 方法：①溶血实验：取新西兰大白兔静脉血，分别加到TiO₂-生理盐水、TiO₂:C-生理盐水、蒸馏水和生理盐水中。②细胞毒性实验：将TiO₂:C浸提液加入对数生长期的L929 细胞培养基中。③短期全身毒性实验：将60只BALB/C小白鼠随机分为3组，分别灌胃给予TiO₂浸提液、TiO₂:C浸提液及生理盐水。④口腔黏膜刺激实验：将牙胶圆片与TiO₂:C纳米薄膜涂片分别缝合固定于仓地鼠颊黏膜上。 结果与结论：纳米TiO₂:C涂层未引起急性溶血，毒性等级为0-1 级，未对细胞产生明显毒性，细胞周期未见明显异常；TiO₂:C浸提液对小鼠无短期全身毒性；对仓鼠口腔黏膜无刺激性。表明纳米TiO₂:C薄膜涂层具有良好的生物安全性。

Biocompatibility of nano-TiO2:C thin film coating

BACKGROUND:In previous experiments, TiO₂:C thin film coating have been prepared on the surface of titanium paper by sol-gel process. OBJECTIVE:To evaluate the biocompatibility of nanometer TiO₂:C thin film coating. METHODS:Hemolysis test: New Zealand white rabbit venous blood was extracted and added into the TiO₂-normal saline, TiO₂:C-normal saline, distilled water and fresh normal saline. Cytotoxicity test: TiO₂:C leaching liquor was added into the L929 cell culture medium in the logarithmic growth phase. Short-term systemic toxicity test: 60 BALB/C mice were randomly divided into three groups and lavaged with leaching liquor extracted from TiO₂, TiO₂:C coatings and normal saline. Oral mucosa stimulation test: the gutta-percha wafer and the nano-TiO2:C thin film coating were sutured and fixed to the cheek pouch of inaurate hamster. RESULTS AND CONCLUSION:Acute hemolysis was not induced by nano-TiO₂:C thin film coating. Toxicity level of nano-TiO₂:C thin film coating was 0-1. It had no obvious toxicity to the cells. There was no obvious abnormality of cell cycle. TiO₂:C leaching liquor had no short-term systemic toxicity on mice and no stimulation to oral mucosa of hamster. The results showed that nano-TiO₂:C thin film coatings have good bio-security.