细胞自噬与结肠癌Lovo细胞迁移和侵袭的相关性研究 |
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作者姓名: | 王天宝 石汉平 韩方海 董文广 |
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作者单位: | 1. 510080 广州,中山大学附属第一医院外科 |
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基金项目: | 广东省科技计划资助项目(2012B060900100) |
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摘 要: | 目的探讨不同自噬状态对结直肠癌Lovo细胞迁移和侵袭能力的影响。
方法将Lovo细胞分为自噬增强组、正常细胞组和3-甲基嘌呤自噬抑制组。用激光共聚焦显微镜观察绿色荧光颗粒,用Q-PCR检测Beclin1 mRNA表达水平,透射电镜观察自噬溶酶体,Transwell实验评价Lovo细胞迁移与侵袭能力。
结果绿色荧光颗粒数以自噬增强组最多,其次为正常细胞组,而自噬抑制组最少。Beclin1 mRNA表达量自噬增强组为(1.23±0.12)个,正常细胞组为(1±0.13)个,自噬抑制组为(0.98±0.1)个。自噬增强组可见大量的自噬溶酶体,明显多于正常细胞组和自噬抑制组。迁移实验细胞计数:自噬增强组高于正常细胞组(138.0±16.7与90.7±12.9,P=0.026)和自噬抑制组(138.0±16.7与92.7±26.7,P=0.030)。侵袭实验细胞计数:自噬增强组高于正常细胞组(147.0±13.0与99.0±20.5,P=0.028)和自噬抑制组(147.0±13.0与95.7±25.6,P=0.021)。
结论结肠癌Lovo细胞自噬增强促进肿瘤细胞迁移和浸润,可能是局部浸润和远处转移的机制之一。
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关 键 词: | 自噬 细胞运动 淋巴细胞,肿瘤浸润 Lovo细胞 |
收稿时间: | 2013-01-06 |
The relationship of cell autophagy and Lovo cell migration and invasion |
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Authors: | Tian-bao WANG Han-ping SHI Fang-hai HAN Wen-guang DONG |
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Institution: | 1. Department of Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China |
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Abstract: | ObjectiveTo investigate the influence of autophagy on migration and invasion in colon cancer cells.
MethodsThe Lovo cells of colon cancer were classified as autophagy-enhanced group, normal cell group, and 3-Methyladenine autophagy-inhibited group.Laser canning confocal microscope was used to identify the green fluorescence particles.Beclin1 mRNA expressive level was measured with Q-PCR.Transmission electron microscope was employed to observe the autolysosome.Transwell chamber was applied to evaluate the migration and invasion of Lovo cell.
ResultsThere were more green fluorescence particles in the autophagy-enhanced group than the normal cell group and the autophagy-inhibited group.The Beclin 1 mRNA expressive level was 1.23±0.12 in the autophagy-enhanced group, while 1±0.13 in the normal cell group and 0.98±0.1 in the autophagy-inhibited group.There were the most autolysosomes in autophagy-enhanced group of all groups.The migration experiment showed the mean number of Lovo cell in the autophagy-enhanced group(138.0±16.7)were more than the normal Lovo cell group(90.7±12.9, P=0.026)and the autophagy-inhibited group(92.7±26.7, P=0.030). The invasion experiment found the mean number of Lovo cell in autophagy-enhanced group(147.0±13.0)were also more than the normal Lovo cell group(99.0±20.5, P=0.028)and the autophagy-inhibited group(95.7±25.6, P=0.021).
ConclusionsAutophagy enhancement could promote the migrating and invasive ability of Lovo cell.And that might be an important mechanism of invasion and metastasis in malignant tumors. |
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Keywords: | Autophagy Cell movement Lymphocytes tumor-infiltrating Lovo cell |
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