骨髓间充质干细胞对再生障碍性贫血患者T细胞的免疫抑制

背景：骨髓间充质干细胞对再生障碍性贫血患者T细胞增殖的影响国内报道较少,而骨髓间充质干细胞是否通过抑制T细胞增殖实现对再生障碍性贫血患者的免疫调节目前尚无定论。目的：观察人骨髓间充质干细胞对再生障碍性贫血患者T细胞的免疫调节作用。方法：体外分离培养、扩增人骨髓间充质干细胞并通过形态学特征以及流式细胞术进行表面标志鉴定,将骨髓间充质干细胞分别与正常人和再生障碍性贫血患者外周血提取的T淋巴细胞共培养7 d。应用ELISA法检测各培养上清液中T淋巴细胞分泌的白细胞介素2、γ-干扰素、白细胞介素4及白细胞介素10水平。结果与结论：再生障碍性贫血组患者培养上清液中T淋巴细胞分泌的白细胞介素2、γ-干扰素水平明显高于正常人(P < 0.05),白细胞介素4、白细胞介素10低于正常人(P < 0.05)。骨髓间充质干细胞可下调白细胞介素2、γ-干扰素表达,同时上调白细胞介素4、白细胞介素10表达,从而调节再生障碍性贫血患者的免疫紊乱。

Immunosuppression of bone marrow mesenchymal stem cells on T cells in patients with aplastic anemia

BACKGROUND: To date, there are few domestic reports about the influence of bone marrow mesenchymal stem cells on T cells proliferation in patients with aplastic anemia. And no study addresses the topic that if bone marrow mesenchymal stem cells achieve immune regulation in aplastic anemia patients through inhibiting T cells proliferation. OBJECTIVE: To explore the effects of human bone marrow mesenchymal stem cells on T cells immune regulation in patients with aplastic anemia.METHODS: Human bone marrow mesenchymal stem cells were isolated, cultured and subcultivated in vitro. The morphological appearance of bone marrow mesenchymal stem cells was observed and surface markers were measured by flow cyometry. The bone marrow mesenchymal stem cells were co-cultured with T cells extracted from peripheral blood of healthy volunteers and aplastic anemia patients for 7 days. The expressions of interferon-γ, interleukin-4 and interleukin-10 in the supernatants were detected with enzyme linked immunosorbent assay.RESULTS AND CONCLUSION: The levels of interleukin-2 and interferon-γ in the supernatant of aplastic anemia patients were significantly higher (P < 0.05), while levels of interleukin-4 and interleukin-10 were significantly lower than that in healthy controls (P < 0.05). Bone marrow mesenchymal stem cells suppressed the elevated levels of interleukin-2 and interferon-γ, and enhanced the decreased levels of interleukin-4 and interleukin-10, thus regulating the immune dysfunction of aplastic anemia patients.