储运条件对脐带间充质干细胞存活率的影响

背景：国内外尚无对脐带间充质干细胞运输过程中相关活力指标的全面评估。 目的：检测白蛋白和储运时间等不同条件对脐带间充质干细胞存活率的影响。 方法：体外培养的脐带间充质干细胞分为实验组和对照组，每组将3.15×10⁹ L^-1细胞悬浮在3 mL生理盐水中。其中实验组加入1%白蛋白并避光保存，对照组分为不加白蛋白避光保存组和加白蛋白光照保存组，在0，2，4，8和24 h分别测定各组细胞数、锥虫蓝拒染率和细胞存活率。 结果与结论：与对照组相比，实验组(保存液中加白蛋白且避光)的8 h细胞损失率几乎为零，细胞存活率接近90%。对照中不加白蛋白组8 h细胞损失率高达38.5%，细胞存活率为86%。在脐带间充质干细胞的长途运输中，加入1%白蛋白可以有效保证细胞存活量。运输时间超出8 h后，细胞损失率上升且存活率下调。实验数据表明，16 ℃、避光和1%白蛋白生理盐水的保存条件下，脐带间充质干细胞在8 h内符合临床输注要求。

Effects of transportation conditions on survival rate of umbilical cord mesenchymal stem cells

BACKGROUND: General assessment of viability of umbilical cord mesenchymal stem cells during transport is not considered at home and abroad.    OBJECTIVE: To explore the effects of different transportation conditions such as albumin and transport time on survival rate of umbilical cord mesenchymal stem cells.  METHODS:Umbilical cord mesenchymal stem cells cultured in vitro were divided into experimental and control groups. Each of group contained 3.15×10⁹/L cells in 3 mL normal saline. The experimental group contained 1% albumin in dark environment. The control group included two subgroups: dark preservation group with the absence of albumin and light preservation group with the presence of albumin. Cell counting, trypan blue staining and cell survival rate were compared at 0, 2, 4, 8, and 24 hours.  RESULTS AND CONCLUSION: Compared with the control group, no cell loss was observed in experimental group (with the presence of albumin in the dark) and a 90% viability ratio was achieved at 8 hours. In the control group without albumin, loss rate reached 38.5% and survival rate reached 86% at 8 hours. Results revealed that 1% albumin predominantly improved cell survival rate in long-distance transport of umbilical cord mesenchymal stem cells. When transport time was more than 8 hours, cell loss rate increased and cell survival rate downregulated. Our experimental data demonstrated that umbilical cord mesenchymal stem cells preserved in normal saline consisting of 1% albumin placing in dark environment at 16 ℃ apply to clinical application criterion in 8 hours.