基因修饰骨髓间充质干细胞修复关节软骨损伤

背景：随着生物技术的发展,通过转基因技术修饰细胞,从而获得长期稳定表达的生物活性因子以治疗关节软骨损伤逐渐引起重视。目的：就基因修饰的骨髓间充质干细胞在修复关节软骨损伤中的应用作一综述。方法：由第一作者检索1990至2011年PubMed数据库(http://www.ncbi.nlm.nih.gov/PubMed)有关基因修饰骨髓间充质干细胞修复关节软骨损伤的文献,英文检索词为“cartilage,gene therapy,mesenchymal stem cells,tissue engineering,bioactive factor,vector”。共纳入15篇文献归纳总结。结果与结论：骨髓间充质干细胞已被广泛应用于修复关节软骨损伤。通过转基因技术将特定外源基因导入骨髓间充质干细胞,联合细胞治疗和基因治疗可达到更好的治疗效果,在关节软骨损伤的治疗中有广阔的应用前景。

Gene-modified bone marrow mesenchymal stem cells for repair of articular cartilage injury

BACKGROUND:Bone marrow mesenchymal stem cells have the potential of self-renewal and multiple differentiations, and can be induced into osteoblasts and chondrocytes under certain conditions, so they have been widely used for articular cartilage repair in recent studies.OBJECTIVE:To review application of gene-modified bone marrow mesenchymal stem cells in repair of articular cartilage injury.METHODS:A computer-based online retrieval of PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) was performed by the first author to search papers regarding gene-modified bone marrow mesenchymal stem cells for repair of articular cartilage injury using the English key words “cartilage, gene therapy, mesenchymal stem cells, tissue engineering, bioactive factor, vector”. After excluding papers with nonrelated and repetitive contents, 15 papers were suitable for final analysis.RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells have been widely used for repair of articular cartilage injury. Bone marrow mesenchymal stem cells with specific exogenous gene introduced by transgenic technology could acquire better therapeutic effects in treatment of articular cartilage injury.