幼鼠反复癫痫发作致苔藓纤维发芽的动态变化及其与自发性发作的关系

目的 观察幼鼠反复癫痫发作致苔藓纤维发芽(MFS)的动态变化及其与慢性期反复自发性发作(SRS)的关系。方法 将190只幼年期Wistar大鼠随机分为4组：对照组(n=48)、EP1组(n=80)、空白组(n=12)和EP2组(n=50)，应用氯化锂-匹罗卡品致痫幼鼠模型，诱导幼鼠在生后21、25、29d反复癫痫发作，采用Timm染色法观察EP1组幼鼠末次癫痫发作后1、3、7、14、20、30、45、60d海马MFS的动态变化；观察EP2组SRS情况，比较EP2组中出现SRS(EP2-SRS组)和未出现SRS(EP2-非SRS组)的大鼠MFS的差异；采用Nissl染色观察海马神经元坏死及凋亡情况，对CA3、CA1区神经元进行计数，观察SRS对海马神经元的影响。结果 Timm染色显示，与对照组相比，EP1组在反复癫痫发作后14d MFS明显增加，并持续至60d后(P<0.05)，EP2-SRS组与EP2-非SRS组相比无明显差异(P<0.05)。EP2-SRS组与空白组相比，CA3、CA1区神经元损伤明显(P<0.05)。结论 幼年反复癫痫发作引起MFS明显增加；在氯化锂-匹罗卡品模型中，MFS可能不是导致SRS的因素。

Dynamic changes of mossy fiber sprouting caused by seizures in immature rats and its relationship with spontaneous recurrent seizures

Objective To investigate the changes of mossy fiber sprouting(MFS) in hippocampus of rats which suffered lithium-pilocarpine induced repeatedly seizures, and the correlation between MFS and spontaneous recurrent seizures(SRS). Methods One hundred and ninety immature Wistar rats were randomly divided into four groups: control group(n=48), EP1 group(n=80), blank group(n=12), EP2 group(n=50). Lithium-pilocarpine was used to induce epilepsy model of rats. Rats were given intraperitoneal injections of pilocarpine on P21, P25 and P29 to induce recurrent seizures. The changes of MFS in hippocampus were examined on days 1, 3, 7, 14, 20, 30, 45 and 60 by Timm staining. Rats in EP2 group were observed every day and were subdivided into EP2-SRS and EP2-nonSRS group, then the difference in MFS was compared between the two groups. The necrosis and apoptosis of the neurons in hippocampus were observed and the neurons in CA3 and CA1 areas were counted by Nissl staining, thus the effect of SRS on the hippocampal neurons could be assessed. Results Compared with control group, significant MFS phenomenon was detected from day 14 to day 60 after recurrent seizures in EP1 group(P<0.05). There was no difference in MFS between EP2-SRS and EP2-nonSRS groups. Compared with blank group, prominent neuron loss was observed in CA3 and CA1 regions of the rats with SRS(P<0.05). Conclusion Recurrent seizures in immature rats can cause an increase of MFS in the hippocampus. We suggest a dissociation between SRS and MFS in the lithium-pilocarpine model of epilepsy.