癫痫持续状态大鼠海马线粒体分裂、融合的变化

目的 探讨癫痫持续状态大鼠海马线粒体分裂、融合的变化。方法 将50只Wistar大鼠随机分为正常对照组和癫痫组，癫痫组分为癫痫持续状态后4、8、24和72h组，观察癫痫持续状态后不同时间点大鼠海马线粒体分裂、融合的变化。Western blotting、PCR分析海马线粒体中Drp1、hFis1、 Mfn1、Opa1的表达；免疫组化分析海马CA3区神经细胞Drp1、Opa1的表达。结果 Drp1、 hFis1在癫痫持续状态后4h时开始升高，24h达高峰，72h仍处于较高水平；Mfn1、Opa1在癫痫持续状态后4h时出现短暂的升高，随即表达下降，并于24h时达最低水平。癫痫持续状态后24h时，大鼠海马CA3区Drp1阳性神经元数目显著高于对照组(P＜0.05)，Opa1阳性神经元数目显著低于对照组(P＜0.05)。结论 癫痫持续状态大鼠海马线粒体分裂、融合失衡，主要表现为线粒体分裂增强、线粒体融合抑制。

Alteration of mitochondrial fission and fusion in hippocampus of rats with status epilepticus

Objective To investigate the alteration of mitochondrial fission and fusion in hippocampus of rats with status epilepticus (SE). Methods Male Wistar rats were randomly divided into five groups：normal control group and epileptic group (4, 8, 24 and 72 h after SE). The alteration of mitochondrial fission and fusion in hippocampus of rats were evaluated at different time points after SE. Western blotting and PCR were used to estimate the expression of dynamin related protein1 (Drp1), human mitochondrial fission protein1 (hFis1), mitofusin1 (Mfn1) and optic atrophy1 gene protein1 (Opa1). Immunohistochemistry was used to estimate the expressions of Drp1 and Opa1 in neurons of hippocampal CA3 region 24 h after SE. Results Mitochondrial fission proteins Drp1and hFis1 began to increase 4 h after SE, with a peak at 24 h and still elevated 72 h after SE. Whereas mitochondrial fusion proteins Mfn1and Opa1 increased transiently at 4 h after SE and decreased sharply thereafter, falling into the bottom at 24 h. The number of neurons expressed Drp1 at 24 h after SE was significantly higher than that in normal control group (P＜0.05), while the number of neurons expressed Opa1 was significantly lower than that in normal control group(P＜0.05). Conclusion SE leads to imbalanced mitochondrial fission and fusion, shown by the enhanced mitochondrial fission and the declined mitochondrial fusion.