骨形态发生蛋白2/注射式硫酸钙缓释载体的生物相容性

背景：目前硫酸钙主要被作为抗生素载体和成骨因子载体。 目的：观察骨形态发生蛋白2/注射式硫酸钙缓释系统的体外缓释效果及与种子细胞的生物相容性。 方法：制备骨形态发生蛋白2/注射式硫酸钙缓释系统,检测其体外释放性能。将SD大鼠骨髓间充质干细胞经体外诱导培养、扩增后,种植于骨形态发生蛋白2/注射式硫酸钙缓释载体和单纯的注射式硫酸钙支架上行体外培养。 结果与结论：骨形态发生蛋白2/注射式硫酸钙缓释支架具有缓释骨形态发生蛋白2的效果,持续时间可达31 d。骨形态发生蛋白2/注射式硫酸钙缓释支架与大鼠骨髓间充质干细胞具有优良的生物相容性,并且相同时间点支架上的细胞黏附率、细胞数量及细胞碱性磷酸酶活性均明显高于注射式硫酸钙支架;扫描电镜发现两组材料上均有细胞生长,骨形态发生蛋白2/注射式硫酸钙缓释支架上的细胞在支架表面和孔隙内生长良好,细胞突起接触融合,细胞密集区域可见细胞外基质形成,大量细胞包绕在材料表面;注射式硫酸钙支架上的细胞黏附数量较少,生长情况不及骨形态发生蛋白2/注射式硫酸钙缓释支架上的细胞。

Biocompatibility of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release carriers

BACKGROUND: The calcium sulfate is mostly used as carriers for antibiotics and osteogenic factors by now.  OBJECTIVE: To observe the in vitro delivery effect of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release system and the in vitro biocompatibility with bone marrow mesenchymal stem cells. METHODS: Bone morphogenetic protein-2/injectable calcium sulfate cement slow-release scaffold was prepared and its release properties were detected. Bone marrow mesenchymal stem cells obtained from Sprague-Dawley rats were induced, cultured and proliferated in vitro, and then, the cells were seeded onto the bone morphogenetic protein-2/injectable calcium sulfate cement scaffolds (experimental group) or single injectable calcium sulfate cement scaffolds (control group). RESULTS AND CONCLUSION:The release of bone morphogenetic protein-2 on the scaffolds of experimental group could last for 31 days. Rat bone marrow mesenchymal stem cells had a good biocompatibility with the bone morphogenetic protein-2/injectable calcium sulfate cement scaffolds. The adhesive rate, cell number and alkaline phosphatase activity at the same time point in the scaffolds of experimental group were significantly greater than those of the control group. Cells could be observed on each scaffold under a scanning electron microscope. In the experimental group, interconnected cell processes were fused, extracellular matrix was visible in the dense area, and the scaffold was surrounded by a large number of cells. The cells in experimental group grew better than the control group.