提高激素性股骨头坏死模型成活率的方法

背景：激素性股骨头坏死动物模型建立过程中动物的死亡成为影响实验结果的主要因素。目的：观察以脂多糖和地塞米松建立股骨头坏死模型,提高模型成功率的方法。方法：新西兰大白兔48只随机等分为模型组、庆大霉素组、庆大霉素+兰索拉唑组和对照组,前3组连续2 d经耳缘静脉注射脂多糖,再连续3 d,臀部肌肉注射地塞米松注射液建立股骨头坏死模型,庆大霉素组和庆大霉素+兰索拉唑组在建模成功后连续7 d以庆大霉素灌胃,同时庆大霉素+兰索拉唑组肌肉注射兰索拉唑注射液。对照组兔仅注射生理盐水。结果与结论：模型组、庆大霉素组和庆大霉素+兰索拉唑组均造模成功;其中庆大霉素+兰索拉唑组存活状况最好,3组动物死亡率分别是33.3%,25%,8.3%,模型组、庆大霉素组、庆大霉素组+兰索拉组和对照组兔的死亡数量差异有显著性意义(P < 0.05)。提示在兔激素性股骨头坏死模型建立中,庆大霉素+兰索拉唑的使用,可以提高实验动物的生存率。中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程全文链接：

A method for elevating survival rate of models of steroid-induced necrosis of femoral head

BACKGROUND: Animal death is a main influential factor for experimental results in establishment of animal models of steroid-induced necrosis of femoral head. OBJECTIVE: To observe models of femoral head necrosis established using lipopolysaccharide and dexamethasone so as to elevate success rate of model induction. METHODS: A total of 48 New Zealand white rabbits were equally and randomly divided into model group, gentamicin group, gentamicin + lansoprazole group and control group. The first three groups were injected with lipopolysaccharide for 2 consecutive days via the ear vein, and then they were injected with dexamethasone via intramuscular injection in the buttocks for 3 consecutive days to establish models of femoral head necrosis. The rabbits of gentamicin group, gentamicin + lansoprazole group were intragastrically administered gentamicin for 7 consecutive days after success model induction. Simultaneously, gentamicin + lansoprazole group received intramuscular injection with lansoprazole. Rabbits in the control group were only injected with saline. RESULTS AND CONCLUSION: Models were successfully established in the model, gentamicin, gentamicin + lansoprazole groups. Their conditions were best in the gentamicin + lansoprazole group. Mortalities in above-mentioned groups were 33.3%, 25% and 8.3%, respectively. Significant differences in the number of dead rabbits were detected in the model, gentamicin, gentamicin + lansoprazole and control groups (P < 0.05). Results indicated that the combined use of gentamicin and lansoprazole can elevate survival rate of experimental animals during the establishment of rabbit models of steroid-induced necrosis of femoral head.